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首页> 外文期刊>ACS applied materials & interfaces >Fabrication of Hemin-Doped Serum Albumin-Based Fibrous Scaffolds for Neural Tissue Engineering Applications
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Fabrication of Hemin-Doped Serum Albumin-Based Fibrous Scaffolds for Neural Tissue Engineering Applications

机译：用于神经组织工程应用的血葱掺杂血清白蛋白基纤维支架的制备

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Neural tissue engineering (TE) represents a promising new avenue of therapy to support nerve recovery and regeneration. To recreate the complex environment in which neurons develop and Mature, the ideal biomaterials for neural TE require a number of properties and capabilities including the appropriate biochemical and physical cues to adsorb and release specific grOwth factors. Here, we present neural TE constructs based on electrospun serum albumin (SA) fibrous scaffolds. We doped out SA scaffolds with,an iron-containing porphyrin, hemin, to confer conductivity, and then-functionalized them with: different recombinant proteins and growth factors to ensure cell attachment and proliferation. We demonstrated the potential for these constructs combining topographicalkbiochemical, and electrical stimuli by testing them with clinically relevant neural populations derived from human induced pluripotent stem cells (hiPSCs). Our scaffolds could support the attachment, proliferation, and neuronal differentiation of hiPSC-derived neural stem cells (NSCs), and were also able to incorporate.active growth factors and release them over time, which modified the behavior of cultured cells and substituted the need for grOwth factor supplementation by media change. Electrical stimulation on the doped SA scaffold positively influenced the maturation of neuronal populations, with neurons exhibiting more branched neurites compared to controls. Through promotion of; cell proliferation, differentiation, and neurite branching of hiPSC-derived NSCs, these conductive SA fibrous scaffolds are of broad application in nerve regeneration strategies.
机译：神经组织工程（TE）代表了有希望的新疗法，以支持神经恢复和再生。为了重建神经元发展和成熟的复杂环境，神经TE的理想生物材料需要许多性质和能力，包括适当的生化和物理提示来吸附和释放特异性生长因子。在这里，我们介绍基于Electrom血清白蛋白（SA）纤维支架的神经TE构建体。我们用含铁的卟啉，血红素掺杂出SA支架，以赋予电导率，然后 - 用：不同的重组蛋白和生长因子官能化，以确保细胞附着和增殖。我们证明了这些构建体的潜力，通过用源自人诱导多能干细胞（HIPSC）的临床相关的神经群体来将拓扑基化学和电刺激组合。我们的支架可以支持HIPSC衍生的神经干细胞（NSCs）的附着，增殖和神经元分化，并且还能够掺入。活性生长因子并随时间释放它们，这改性了培养细胞的行为并取代了需求通过媒体变化补充生长因子。掺杂SA支架上的电气刺激积极影响神经元群的成熟，与对照相比，神经元表现出更多的分支神经肌腱。通过推广;髋关节衍生NSCs的细胞增殖，分化和神经突支化，这些导电SA纤维支架具有广泛的神经再生策略应用。

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