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首页> 外文期刊>ACS applied materials & interfaces >Fabrication of Stand-Alone Cell-Laden Collagen Vascular Network Scaffolds Using Fugitive Pattern-Based Printing-Then-Casting Approach
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Fabrication of Stand-Alone Cell-Laden Collagen Vascular Network Scaffolds Using Fugitive Pattern-Based Printing-Then-Casting Approach

机译：使用基于逃逸的图案的印刷铸造方法制造独立细胞载胶血管网络支架

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摘要

Vascular networks are of great significance in tissue engineering and viewed as the first step to fabricate human tissues. Although various techniques have been investigated to create vascular and vascular-like networks, the fabrication of stand-alone pure collagen based vascular constructs is still a challenge because of the poor extrudability, weak mechanical property, and long cross-linking time of pure collagen solutions. In this study, a fugitive pattern-based printing then-casting approach is investigated. The proposed alginate-based fugitive ink has excellent mechanical strength (by adding Laponite nanoclay), printability (by adding Laponite nanoclay), and controllable gelation rate (by adding disodium hydrogen phosphate). Using this fugitive ink, complex vascular-like structures can be easily printed and cross-linked in Laponite EP bath as fugitive vascular tree patterns. Each fugitive vascular tree pattern is then embedded in a gelatin bath to make a gelatin mold with the tree patterns. With the help of sodium citrate, the fugitive vascular tree pattern is liquefied and removed to create the gelatin mold with vascular channels. Finally, a stand-alone collagen vascular network scaffold embedded with fibroblasts can be fabricated by casting the cell-laden collagen suspension into the gelatin mold and releasing it from the mold at 37 degrees C. The cell-related investigations indicate that the cells grow and spread well in the pure collagen vascular network scaffold. The proposed hybrid printing-then-casting approach also provides a feasible technology to fabricate with materials having low viscosity, long gelation time, and poor mechanical property.
机译：血管网络在组织工程方面具有重要意义，并视为制造人组织的第一步。虽然已经研究了各种技术以产生血管和血管状网络，但是由于耐凝固性，弱的机械性能和纯胶原溶液的长交联时间，因此独立的纯胶原基的血管构建体的制造仍然是一个挑战。在该研究中，研究了一种逃逸的基于图案的印刷然后铸造方法。所提出的基于藻酸盐的逃逸油墨具有优异的机械强度（通过加入Laponite NaNIClay），可印刷性（通过加入Laponite NaNIClay）和可控凝胶化率（通过添加磷酸二钠）。使用这种逃逸油墨，可以容易地印刷和交联在Laponite EP浴中作为逃逸血管树图案交联。然后将每个逃逸血管树图案嵌入明胶浴中，使明胶模具具有树木图案。借助柠檬酸钠，逃逸血管树图案是液化并除去以产生具有血管通道的明胶模具。最后，可以通过将细胞升起的胶原蛋白悬浮液浇铸到明胶模具中并在37℃下将其从模具释放来制造嵌入成纤维细胞的独立胶原血管网络支架。细胞相关的研究表明细胞增长在纯胶原膜血管网络脚手架中展开。所提出的杂交印刷铸造方法还提供了一种可行的技术，用于制造具有低粘度，长凝胶时间和机械性质差的材料。

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