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首页> 外文期刊>ACS applied materials & interfaces >Three-Dimensional Hierarchical Nanofibrous Collagen Scaffold Fabricated Using Fibrillated Collagen and Pluronic F-127 for Regenerating Bone Tissue
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Three-Dimensional Hierarchical Nanofibrous Collagen Scaffold Fabricated Using Fibrillated Collagen and Pluronic F-127 for Regenerating Bone Tissue

机译：使用原纤化胶原和Pluronic F-127制造三维等级纳米纤维胶原支架，用于再生骨组织

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摘要

It is well known that a nanoscale fibrous structure can provide a unique stage for encouraging reasonable cell activities including attachment and proliferation owing to its similar topological structure to the extracellular matrix. Hence, the structure has been widely applied in tissue regeneration. Type-I collagen has been typically used as a typical tissue regenerative material owing to its biocompatibility and abundance, although it has potential for antigenicity. In particular, collagen has been fabricated in two different forms, porous spongy and nanofibers. However, although the structures provided outstanding cellular activities, they exhibit disadvantages such as low cell migration capabilities in a spongy scaffold owing to the low degree of interconnected macropores and low processability in fabricating three-dimensional (3D) structures in an electrospun collagen scaffold. Hence, the fabrication of 3D nanofibrous collagen structures with interconnected macropores can be extremely challenging. In this work, we developed a 3D collagen scaffold consisting of multilayered nanofibrous struts fabricated using a 3D printing process and pluronic F-127 (PF-127), which is a thermoreversible polymer. After optimizing various processing conditions, we successfully achieved the 3D nanofibrous collagen mesh structure with fully interconnected macropores. A 3D printed collagen scaffold that was fabricated using a low-temperature printing process was applied as a control. Through various analyses using physical properties (surface morphology, fibronectin absorption, mechanical properties, etc.) and cell activities using preosteoblasts (MC3T3-E1), we are convinced that the newly designed 3D nanofibrous collagen scaffold can be a new promising scaffold for bone tissue engineering.
机译：众所周知，纳米级纤维结构可以提供一种独特的阶段，用于鼓励合理的细胞活性，包括由于其与细胞外基质类似的拓扑结构而存在连接和增殖。因此，该结构已广泛应用于组织再生。由于其生物相容性和丰度，型I型胶原蛋白通常用作典型的组织再生材料，尽管它具有抗原性潜力。特别是，胶原蛋白已以两种不同的形式制造，多孔海绵状和纳米纤维制造。然而，尽管结构提供了优异的蜂窝活性，但由于互联的大孔的互联的大孔和在电纺器胶原支架中制造三维（3D）结构的低可加工性，它们在海绵状脚手架中表现出诸如低细胞迁移能力的缺点。因此，用互连的大孔的3D纳米纤维胶原结构的制造可能非常具有挑战性。在这项工作中，我们开发了一种由使用3D印刷工艺和Pluronic F-127（PF-127）制造的多层纳米纤维支柱组成的3D胶原支架，其是热可逆聚合物。在优化各种加工条件之后，我们成功地实现了具有完全互联的宏孔的3D纳米纤维胶原网结构。使用低温印刷方法制造的3D印刷胶原屑支架作为对照。通过各种分析使用物理性质（表面形态，纤维连接蛋白吸收，机械性能等）和使用预卵细胞（MC3T3-E1）的细胞活性，我们相信新设计的3D纳米纤维胶原屑脚手架可以是骨组织的新有前途的支架工程。

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