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首页> 外文期刊>ACS applied materials & interfaces >Hydrodynamic Layer-by-Layer Assembly of Transferable Enzymatic Conductive Nanonetworks for Enzyme-Sticker-Based Contact Printing of Electrochemical Biosensors
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Hydrodynamic Layer-by-Layer Assembly of Transferable Enzymatic Conductive Nanonetworks for Enzyme-Sticker-Based Contact Printing of Electrochemical Biosensors

机译：用于酶 - 贴纸的电化学生物传感器的酶 - 贴纸接触印刷的可转移酶导电纳米纳米型逐层组装

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摘要

Realizing high-performance electrochemical biosensors in a simple contact-printing-based approach significantly increases the applicability of integrated flexible biosensors. Herein, an enzyme-sticker-based approach that enables flexible and multielectrochemical sensors via simple contact-transfer printing is reported. The enzyme sticker consists of an enzymatic conductive network film and a polymeric support. The enzyme-incorporated nanostructured conductive network showing an efficient electrical coupling was assembled via the hydrodynamic layer-by-layer assembly of redox enzymes, polyelectrolytes, single-walled carbon nanotubes, and a biological glue material, M13 phage. The enzymatic conductive network on a polymeric membrane support was facilely wet contact-transfer printed onto integrated electrode systems by exploiting varying degrees of hydrophilicity displayed by the enzymatic electronic film, polymeric support, and receiving electrodes of the sensor system. The glucose sensors fabricated using the enzyme sticker detected glucose at a concentration of as low as 35 mu M and showed high selectivity and stability. Furthermore, a flexible dual-sensor array capable of detecting both glucose and lactate was demonstrated using the versatile enzyme sticker concept. This work presents a new route toward assembling and integrating hybrid nanomaterials with efficient electrochemical coupling for high-performance biosensors and health-monitoring devices as well as for emerging bioelectronics and electrochemical devices.
机译：以简单的接触印刷方法实现高性能电化学生物传感器显着提高了集成柔性生物传感器的适用性。这里，报道了一种基于酶 - 贴纸的方法，其通过简单的接触转移印刷能够实现柔性和多电化学传感器。酶贴纸由酶导电网络膜和聚合物载体组成。通过氧化还原酶，聚电解质，单壁碳纳米管和生物胶材料，M13噬菌体，M13噬菌体，通过逐层组装酶掺入的纳米结构导电网络。聚合物膜载体上的酶导电网络通过利用由酶电子膜，聚合物支撑和传感器系统的接收电极显示的不同程度的亲水性印刷到集成电极系统上。使用酶贴纸检测到浓度低至35μm的葡萄糖制造的葡萄糖传感器，并显示出高选择性和稳定性。此外，使用通用酶贴纸概念来证明能够检测葡萄糖和乳酸盐的柔性双传感器阵列。该工作介绍了具有高效生物传感器和健康监测装置的有效电化学耦合的杂交纳米材料的组装和整合杂种纳米材料的新路线以及新兴生物电子和电化学装置。

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