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首页> 外文期刊>ACS applied materials & interfaces >Transpiration-Inspired Fabrication of Opal Capillary with Multiple Heterostructures for Multiplex Aptamer-Based Fluorescent Assays
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Transpiration-Inspired Fabrication of Opal Capillary with Multiple Heterostructures for Multiplex Aptamer-Based Fluorescent Assays

机译：多重异质结构对多重型型荧光测定的多重异质结构的透吹液的制作

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摘要

In this work we report a method for the fabrication of opal capillary with multiple heterostructures for aptamer-based assays. The method is inspired by plant transpiration. During the fabrication, monodisperse SiO2 nanoparticles (NPs) self-assemble in a glass capillary, with the solvent gradually evaporating from the top end of the capillary. By a simple change of the colloid solution that wicks through the capillary, multiple heterostructures can be easily prepared inside the capillary. On the surface of the SiO2 NPs, polydopamine is coated for immobilization of aminomethyl-modified aptamers. The aptamers are used for fluorescent detection of adenosine triphosphate (ATP) and thrombin. Owing to fluorescence enhancement effect of the photonic heterstructures, the fluorescent signal for detection is amplified up to 40-fold. The limit of detection is 32 M for ATP and 8.1 nM for thrombin. Therefore, we believe this method is promising for the fabrication of analytical capillary devices for point-of-care testing.
机译：在这项工作中，我们报告了一种用多种异质结构制备蛋白质毛细血管的方法，用于适体的基于型测定。该方法受植物蒸腾的启发。在制造期间，单分散SiO 2纳米颗粒（NPS）在玻璃毛细管中自组装，溶剂从毛细管的顶端逐渐蒸发。通过简单地改变通过毛细血管芯，可以在毛细管内部容易地制备多个异质结构。在SiO 2 NPS的表面上，涂覆多碳胺用于固定氨基甲基改性的适体。适体用于荧光检测腺苷三磷酸（ATP）和凝血酶。由于光子捕获结构的荧光增强效果，用于检测的荧光信号高达40倍。对于凝血酶，检测限为32μm，凝血酶为8.1nm。因此，我们认为这种方法很有希望用于制造分析毛细管装置以进行护理点测试。

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  • 来源
    《ACS applied materials & interfaces》 |2017年第38期|共6页
  • 作者

    Gao Bingbing; Tang Litianyi; Zhang Dagan; Xie Zhuoying; Su Enben; Liu Hong; Gu Zhongze;

  • 作者单位

    Southeast Univ Sch Biol Sci &

    Med Engn State Key Lab Bioelect Nanjing 210096 Jiangsu Peoples R China;

    Southeast Univ Sch Biol Sci &

    Med Engn State Key Lab Bioelect Nanjing 210096 Jiangsu Peoples R China;

    Southeast Univ Sch Biol Sci &

    Med Engn State Key Lab Bioelect Nanjing 210096 Jiangsu Peoples R China;

    Southeast Univ Sch Biol Sci &

    Med Engn State Key Lab Bioelect Nanjing 210096 Jiangsu Peoples R China;

    Getein Biotech Inc 9 Bofu Rd Nanjing 211505 Jiangsu Peoples R China;

    Southeast Univ Sch Biol Sci &

    Med Engn State Key Lab Bioelect Nanjing 210096 Jiangsu Peoples R China;

    Southeast Univ Sch Biol Sci &

    Med Engn State Key Lab Bioelect Nanjing 210096 Jiangsu Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 化学工业;
  • 关键词

    colloid crystal; fluorescence enhancement; heterostructure; transpiration; capillary;

    机译：胶体晶体;荧光增强;异质结构;蒸腾;毛细血管;

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