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首页> 外文期刊>American Journal of Physiology >Reply to letter to the editor: to D2O or not to D2O? What are the reasons we D2O it at all?
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Reply to letter to the editor: to D2O or not to D2O? What are the reasons we D2O it at all?

机译：回复给编辑的信：到D2O还是不是D2O？ 我们的原因是什么原因？

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to the editor: the estimation of protein synthesis has long been used as an outcome variable to better understand anabolic processes in skeletal muscle. Much of what we have learned about the regulation of muscle mass and protein metabolism has been a result of our ability to estimate synthesis rates under varying experimental conditions ranging from different types of mechanical loading to nutritional interventions.A number of methodological approaches have been developed to assess protein synthesis that use a labeled precursor as a tracer whereby the rate of protein synthesis can be estimated by measuring the appearance of this tracer into muscle protein over time. To achieve this goal, there are a number of "assumptions" that must be considered while using these methodologies (4, 6). We will focus on two of these assumptions in response to the letter to the editor by Wilkinson et al. (19a). The first, and perhaps foremost, assumption is that the relationship of incorporated label is linear with rates of muscle protein synthesis. In other words, any change in the appearance of the label in skeletal muscle is indicative of a change in the rate of protein synthesis. A second assumption, often somewhat overlooked with recent methodological approaches, is that the contribution of amino acid "recycling" is minimal (or at least constant) at the time of the assessment.
机译：对于编辑：蛋白质合成的估计长期以来被用作结果变量，以更好地理解骨骼肌中的合成代谢过程。我们了解到关于肌肉质量和蛋白质代谢的调节的大部分是我们在不同类型的机械加载到营养干预的不同实验条件下估计合成率的结果。已经开发了方法论方法的数量评估使用标记的前体作为示踪剂的蛋白质合成，由此可以通过将该示踪剂的外观随时间测量肌肉蛋白质来估计蛋白质合成速率。为了实现这一目标，必须在使用这些方法（4,6）时必须考虑的许多“假设”。我们将重点关注由Wilkinson等人答复给编辑的信件中的两个假设。 （19a）。首先，尽管如此，假设掺入标签的关系是具有肌肉蛋白质合成率的线性。换句话说，骨骼肌中标签外观的任何变化都表明蛋白质合成率的变化。第二种假设通常稍微忽略了最近的方法方法，是评估时氨基酸“回收”的贡献是最小的（或至少恒定的）。

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