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首页> 外文期刊>American Journal of Physiology >Orexin-A enhances feeding in male rats by activating hindbrain catecholamine neurons
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Orexin-A enhances feeding in male rats by activating hindbrain catecholamine neurons

机译：orexin-a通过激活后脑儿茶酚胺神经元的雄性大鼠饲喂饲养

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摘要

Both lateral hypothalamic orexinergic neurons and hindbrain catecholamin-ergic neurons contribute to control of feeding behavior. Orexin fibers and terminals are present in close proximity to hindbrain cat-echolaminergic neurons, and fourth ventricular (4V) orexin injections that increase food intake also increase c-Fos expression in hindbrain catecholamine neurons, suggesting that orexin neurons may stimulate feeding by activating catecholamine neurons. Here we examine that hypothesis in more detail. We found that 4V injection of orexin-A (0.5 nmol/rat) produced widespread activation of c-Fos in hindbrain catecholamine cell groups. In the A1 and C1 cell groups in the ventro-lateral medulla, where most c-Fos-positive neurons were also dopa-mine beta hydroxylase (DBH) positive, direct injections of a lower dose (67 pmol/200 nl) of orexin-A also increased food intake in intact rats. Then, with the use of the retrogradely transported immunotoxin, anti-DBH conjugated to saporin (DSAP), which targets and destroys DBH-expressing catecholamine neurons, we examined the hypothesis that catecholamine neurons are required for orexin-induced feeding. Rats given paraventricular hypothalamic injections of DSAP, or unconjugated saporin (SAP) as control, were implanted with 4V or lateral ventricular (LV) cannulas and tested for feeding in response to ventricular injection of orexin-A (0.5 nmol/rat). Both LV and 4V orexin-A stimulated feeding in SAP controls, but DSAP abolished these responses. These results reveal for the first time that catecholamine neurons are required for feeding induced by injection of orexin-A into either LV or 4V.
机译：侧面下丘脑脱盐性神经元和后脑服从素 - Ergic神经元有助于控制饲养行为。 orexin纤维和末端均紧邻后褐色猫 - 梯氨酰胺神经元，第四节内（4V）orexin注射素，增加食物摄入量也增加了在后脑儿茶酚胺神经元中的c-fos表达，表明orexin神经元可以通过激活儿茶酚胺神经元刺激饲料。在这里，我们更详细地检查了该假设。我们发现，4V注射尿苷-A（0.5nmol /大鼠）的注射在后脑儿茶酚胺细胞基团中产生了C-FOS的广泛激活。在腹侧髓质中的A1和C1细胞组中，大多数C-FOS阳性神经元也是DOPA-MINEβ羟化酶（DBH）阳性，直接注射较低剂量（67pmol / 200nl）的orexin- A也增加了完整大鼠的食物摄入量。然后，通过使用逆行的传送的免疫毒素，抗DBH与Saporin（DSAP）缀合的抗DBH，其靶向和破坏表达DBH表达的儿茶酚胺神经元，我们检查了芦苇诱导喂养所需的儿茶酚胺神经元的假设。给定副瓣或未缀有的Saporin（SAP）作为对照的副瓣露床注射的大鼠植入4V或侧卧间（LV）套管，并在响应orexin-a（0.5nmol /大鼠）的夜间注射液中进行饲喂。 LV和4V orexin-A在SAP控制中刺激的饲养，但DSAP废除了这些反应。这些结果首次揭示了通过注射orexin-a诱导的进料中所需的儿茶酚胺神经元，进入LV或4V。

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