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首页> 外文期刊>American Journal of Physiology >Metabolic markers of protein maldigestion after a 15N test meal in minipigs with pancreatic exocrine insufficiency
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Metabolic markers of protein maldigestion after a 15N test meal in minipigs with pancreatic exocrine insufficiency

机译：在胰腺外分泌不足的MINIPIG中，在MINIPIG的15N试验膳食后，蛋白质不明显的代谢标志物

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摘要

The effect of pancreatic exocrine insufficiency (PEI) on protein malabsorption is little documented, partly due to methodological barriers. We aimed to validate biomarkers of protein malabsorption using a ~(15)N test meal in a minipig model of PEI. Six pancreatic duct-ligated minipigs were used as a model of PEI and four nonoperated animals as a control. All animals were equipped with an ileocecal reentrant cannula. Minipigs were given a test meal containing [l5N]casein. The PEI animals repeated the test three times, in the absence of any pancreatic enzymes, or after pancreatic substitution at two levels [A or B: 7,500 or 75,000 (lipase) and 388 or 3881 (protease) FIP U]. Heal chyme, urine, and blood were collected postprandially. Nitrogen and ~(15)N were measured in digestive and metabolic pools. We obtained a gradient of ileal protein digestibility from 29 ± 11% in PEI to 89 ± 6% in the controls and a dose-dependent response of enzymes. Insulin and gastric inhibitory poly-peptide secretions were decreased by PEI, an effect that was counteracted with the enzymes at level B. The total recovery of ~(15)N in urinary urea and plasma proteins was 14 ± 5.1% in the control group and decreased to 5.5 ± 2.1% by PEI. It was dose dependency restored by the treatment. Both ~(15)N recovery in plasma and urine were correlated to protein digestibility. We confirm that the ~(15)N transfer in those pools is a sensitive marker of protein malabsorption. Nevertheless, an optimization of the test meal conditions would be necessary in the view of implementing a clinical test.
机译：胰腺外分泌不足（PEI）对蛋白质吸收热物的影响几乎没有记录，部分原因是由于方法障碍。我们旨在使用PEI的MINIPIG模型中的〜（15）n试验膳食验证蛋白质不吸收的生物标志物。将六种胰腺导管连接的微分物用作PEI和四种非合成动物的模型作为对照。所有动物都配备了对侧胞重圈套管。微米饼被含有含有[L5N]酪蛋白的测试粉。 Pei动物在没有任何胰酶的情况下重复测试三次，或在两个水平的胰腺取代后[A或B：7,500或75,000（脂肪酶）和388或3881（蛋白酶）Fip U]。在后施加的愈合表皮，尿液和血液。在消化和代谢池中测量氮气和〜（15）n。在对照中，在PEI中的29±11％的升降蛋白质消化率从29±11％获得了89±6％，并对酶的剂量依赖性反应。 PEI降低了胰岛素和胃抑制多肽分泌物，其抗对照组尿素和血浆蛋白在尿素和血浆蛋白中的总回收率为14±5.1％ PEI减少至5.5±2.1％。它是治疗恢复的剂量依赖性。血浆和尿液中的〜（15）氮酸复苏与蛋白质消化率相关。我们确认在这些池中的〜（15）N转移是蛋白质不吸收的敏感标记。然而，在实施临床试验时，需要优化测试膳食条件。

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