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首页> 外文期刊>Angewandte Chemie >Activation pH and Gating Dynamics of Influenza A M2 Proton Channel Revealed by Single-Molecule Spectroscopy
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Activation pH and Gating Dynamics of Influenza A M2 Proton Channel Revealed by Single-Molecule Spectroscopy

机译：单分子光谱揭示流感M2质子通道的激活pH与甲型型M2质子通道的浇注动力学

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摘要

Because of its importance in viral replication, the M2 proton channel of the influenza A virus has been the focus of many studies. Although we now know a great deal about the structural architecture underlying its proton conduction function, we know little about its conformational dynamics, especially those controlling the rate of this action. Herein, we employ a single-molecule fluorescence method to assess the dynamics of the inter-helical channel motion of both full-length M2 and the transmembrane domain of M2. The rate of this motion depends not only on the identity of the channel and membrane composition but also on the pH in a sigmoidal manner. For the full-length M2 channel, the rate is increased from approximately 190s(-1) at high pH to approximately 80s(-1) at low pH, with a transition midpoint at pH6.1. Because the latter value is within the range reported for the conducting pK(a) value of the His37 tetrad, we believe that this inter-helical motion accompanies proton conduction.
机译：由于其在病毒复制中的重要性，流感的M2质子通道病毒一直是许多研究的重点。虽然我们现在了解其质子传导功能潜在的结构架构，但我们对其构象动态知之甚少，尤其是控制该动作速率的人。在此，我们采用单分子荧光方法来评估全长M2和M2的跨膜结构域的螺旋间通道运动的动力学。该运动的速率不仅取决于通道和膜组合物的身份，还取决于乙状运动的pH值。对于全长M2通道，在低pH下在高pH至大约80s（-1）的速率下从大约80秒（-1）增加，在PH6.1的转变中点以大约80秒（-1）增加。因为后一个值在报告的RIS37 Tetrad的导电PK（a）值的范围内，我们相信这种斜螺旋运动伴随质子传导。

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