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首页> 外文期刊>Analytical chemistry >Localization of Metabolites of Human Kidney Tissue with Infrared Laser-Based Selected Reaction Monitoring Mass Spectrometry Imaging and Silver-109 Nanoparticle-Based Surface Assisted Laser Desorption/Ionization Mass Spectrometry Imaging
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Localization of Metabolites of Human Kidney Tissue with Infrared Laser-Based Selected Reaction Monitoring Mass Spectrometry Imaging and Silver-109 Nanoparticle-Based Surface Assisted Laser Desorption/Ionization Mass Spectrometry Imaging

机译：人肾组织代谢产物的定位与基于红外激光的选定反应监测质谱质谱法和银 - 109基于纳米粒子的表面辅助激光解吸/电离质谱成像

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摘要

Infrared (IR) laser ablation-remote-electrospray ionization (LARESI) platform coupled to a tandem mass spectrometer (MS/MS) operated in selected reaction monitoring (SRM) or multiple reaction monitoring (MRM) modes was developed and employed for imaging of target metabolites in human kidney cancer tissue. SRM or MRM modes were employed to avoid artifacts that are present in full scan MS mode. Four tissue samples containing both cancerous and noncancerous regions, obtained from three patients with renal cell carcinoma (RCC), were imaged. Sixteen endogenous metabolites that were reported in the literature as varying in abundance between cancerous and noncancerous areas in various human tissues were selected for analysis. Target metabolites comprised ten amino acids, four nucleosides and nucleobases, lactate, and vitamin E. For comparison purposes, images of the same metabolites were obtained with ultraviolet (UV) desorption/ionization mass spectrometry imaging (UV-LDI-MSI) using monoisotopic silver-109 nanoparticle-enhanced target ((AgNPET)-Ag-109) in full-scan MS mode. The acquired MS images revealed differences in abundances of selected metabolites between cancerous and noncancerous regions of the kidney tissue. Importantly, the two imaging methods offered similar results. This study demonstrates the applicability of the novel ambient LARESI SRM/MRM MSI method to both investigating and discovering cancer biomarkers in human tissue.
机译：耦合到在选定的反应监测（SRM）或多重反应监测（MRM）模式中耦合到串联质谱仪（MS / MS）的红外线（IR）激光烧蚀 - 远程电镀电离（Laresi）平台并用于成像靶标人肾癌组织中的代谢物。采用SRM或MRM模式来避免以全扫描MS模式存在的工件。成像从肾细胞癌（RCC）的三名患者获得的含有癌性和非癌症区的四种组织样品。选择在文献中报告的十六个内源代谢物，因为各种人体组织中的癌症和非癌症之间的丰富度变化，用于分析。靶代谢物包含十个氨基酸，四种核苷和核碱基，乳酸和维生素E.对于比较目的，使用单同间体银，用紫外（UV）解吸/电离质谱成像（UV-LDI-MSI）获得相同代谢物的图像全扫描MS模式下为109纳米颗粒增强型目标（（Agnpet）-109）。所获得的MS图像显示出肾组织癌症和非癌症区之间所选代谢物丰富的差异。重要的是，两种成像方法提供了类似的结果。本研究表明，新型环境Laresi SRM / MRM MSI方法对人体组织中的癌症生物标志物的适用性。

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