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Direct Observation of Nanoparticles within Cells at Subcellular Levels by Super-Resolution Fluorescence Imaging

机译：通过超分辨率荧光成像直接观察亚细胞细胞内细胞内的纳米颗粒

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摘要

Direct observation of nanoparticles with high spatial resolution at subcellular levels is of great importance to understand the nanotoxicology and promote the biomedical applications of nanoparticles. Super-resolution fluorescence microscopy can break the diffraction resolution limit to achieve spatial resolution of tens of nanometers, making it ideal for highly accurate observation of nanoparticles in the cellular world. In this study, we introduced the employment of super-resolution fluorescence imaging for monitoring nano-particles within cells. Carbocyanine dyes Alexa Flour 647 labeled mesoporous silica nanoparticles (designated as MSNs-AF647) were constructed as the super-resolution imaging nanoplatform in this work as proof of concept. The MSNs-AF647 were incubated with Hela cells, and the nanoparticles within cells were further monitored by super-resolution fluorescence microscopy. The fluorescence images of MSNs-AF647 within cells captured with the super-resolution fluorescence microscopy showed a much higher spatial resolution than that obtained using conventional fluorescence microscopy, showing that super-resolution fluorescence images can provide more accurate information to locate the nanoparticles at the subcellular levels. Moreover, other functional molecules can be easily loaded into the MSNs-AF647 super-resolution imaging nanoplatform, which suggested that super-resolution fluorescence imaging can further be applied to various bioimaging-related areas, such as imaging-guided therapy, with the aid of the MSNs-AF647 nanoplatform. This study demonstrates that super-resolution fluorescence microscopy offers a highly accurate method to study nanoparticles in the cellular world. We anticipate this strategy may further be applied to research areas such as studying the nanotoxicology and optimization of nanoparticle-based bioprobes or drugs by designing new nanostructured materials with multifunctional properties based on MSNs-AF647.
机译：在亚细胞水平下具有高空间分辨率的纳米颗粒的直接观察对于了解纳米毒理学并促进纳米颗粒的生物医学应用，具有重要意义。超分辨率荧光显微镜可以破坏衍射分辨率限制，以实现数十纳米的空间分辨率，使其理想的是对细胞世界中的纳米颗粒的高度准确观察。在本研究中，我们介绍了用于监测细胞内纳米颗粒的超分辨率荧光成像。 Carbocyanine Dyes Alexa粉647标记的介孔二氧化硅纳米粒子（指定为MSNS-AF647）作为本工作中的超分辨率成像纳米片作为概念证明。将MSNS-AF647与HeLa细胞一起温育，通过超分辨率荧光显微镜进一步监测细胞内的纳米颗粒。用超分辨率荧光显微镜捕获的细胞内MSN-AF647的荧光图像显示出比使用常规荧光显微镜获得的空间分辨率更高，表明超分辨率荧光图像可以提供更准确的信息以在亚细胞处定位纳米颗粒水平。此外，其他功能分子可以容易地装载到MSNS-AF647超分辨率成像纳米片上，这表明可借助于借助于诸如成像引导疗法等各种生物分析相关区域的超级分辨率荧光成像。 MSNS-AF647纳米片。该研究表明，超分辨率荧光显微镜，提供了一种高精度的方法来研究蜂窝世界中的纳米颗粒。我们预期该策略可以进一步应用于研究领域，例如通过基于MSNS-AF647的多功能性质设计新的纳米结构材料，研究基于纳米颗粒基生物体或药物的纳米毒性和优化。

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  • 来源
    《Analytical chemistry》 |2019年第9期|共6页
  • 作者

    Chen Shasha; Wang Jie; Xin Bo; Yang Yanbing; Ma Yurou; Zhou Yu; Yuan Liangjie; Huang Zhenli; Yuan Quan;

  • 作者单位

    Wuhan Univ Coll Chem &

    Mol Sci Minist Educ Key Lab Analyt Chem Biol &

    Med Wuhan 430072 Peoples R China;

    Wuhan Univ Coll Chem &

    Mol Sci Minist Educ Key Lab Analyt Chem Biol &

    Med Wuhan 430072 Peoples R China;

    Huazhong Univ Sci &

    Technol Wuhan Natl Lab Optoelect Wuhan 430074 Peoples R China;

    Wuhan Univ Coll Chem &

    Mol Sci Minist Educ Key Lab Analyt Chem Biol &

    Med Wuhan 430072 Peoples R China;

    Wuhan Univ Coll Chem &

    Mol Sci Minist Educ Key Lab Analyt Chem Biol &

    Med Wuhan 430072 Peoples R China;

    Wuhan Univ Coll Chem &

    Mol Sci Minist Educ Key Lab Analyt Chem Biol &

    Med Wuhan 430072 Peoples R China;

    Wuhan Univ Coll Chem &

    Mol Sci Minist Educ Key Lab Analyt Chem Biol &

    Med Wuhan 430072 Peoples R China;

    Huazhong Univ Sci &

    Technol Wuhan Natl Lab Optoelect Wuhan 430074 Peoples R China;

    Wuhan Univ Coll Chem &

    Mol Sci Minist Educ Key Lab Analyt Chem Biol &

    Med Wuhan 430072 Peoples R China;

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  • 正文语种 eng
  • 中图分类 分析化学;
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