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首页> 外文期刊>Analytical chemistry >On-Site Ultrasensitive Detection Paper for Multiclass Chemical Contaminants via Universal Bridge-Antibody Labeling: Mycotoxin and Illegal Additives in Milk as an Example
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On-Site Ultrasensitive Detection Paper for Multiclass Chemical Contaminants via Universal Bridge-Antibody Labeling: Mycotoxin and Illegal Additives in Milk as an Example

机译：通过通用桥抗体标记的多烷化学污染物的现场超敏检测纸：乳突和牛奶中的非法添加剂作为一个例子

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摘要

Multiclass chemical contamination of food has aroused ever-increasing attention due to the increasingly common findings of the co-occurrence of multiclass contamination, such as mycotoxin (aflatoxin M1, AFM1) and illegal additive (melamine, MEL). In the present study, a rapid, ultrasensitive detection paper was developed on the basis of a unique bridge-antibody label to realize on-site simultaneous detection of AFM1 and MEL in milk. This detection paper used the bridge-antibody label on fluorescent particles (i.e., the fluorescent Eu nanoparticles were first conjugated with polyclonal antibodies and then with monoclonal antibodies). Dramatically enhanced sensitivity was found, probably due to the increase in immobilization of efficient monoclonal antibodies onto microspheres. Under optimal conditions, the lower limits of detection were 0.009 and 0.024 ng/mL for AFM1 and MEL in milk, respectively, in comparison with similar works. Moreover, the cutoff values were 0.4 and 150 ng/mL for AFM1 and MEL, respectively. The recoveries ranged from 88.7% to 105.0% for AFM1 and from 84.6% to 117.7% for MEL, with relative standard deviations (RSDs) of 0.5-9.9% during the intraday and interday experiments. Comparison experiments conducted using the detection paper, HPLC, and UPLC-MS/MS found excellent agreement in the simultaneous detection of AFM1 and MEL in milk. This proposed method can be extensively employed for simultaneous monitoring of multiclass chemical contaminants to ensure food safety.
机译：由于多曲霉污染的共同发现，例如霉菌毒素（黄曲霉毒素M1，AFM1）和非法添加剂（三聚氰胺，MEL），因此，多烷化学污染的食物引起了不断增加的关注。在本研究中，基于独特的桥抗体标记开发了一种快速的超敏检测纸，以实现现场同时检测AFM1和MEL在牛奶中的MEL。该检测纸使用荧光颗粒上的桥抗体标记（即，荧光EU纳米粒子首先与多克隆抗体缀合，然后用单克隆抗体缀合）。发现敏感性显着增强了敏感性，可能是由于将有效单克隆抗体固定到微球上的增加。在最佳条件下，与类似作品相比，分别为AFM1和MEL中的检测限为0.009和0.024ng / ml。此外，截止值分别为AFM1和MEL的0.4和150ng / ml。恢复的回收率为AFM1的88.7％至105.0％，对MEL的84.6％至117.7％，在盘中和白天实验期间，相对标准偏差（RSD）为0.5-9.9％。使用检测纸，HPLC和UPLC-MS / MS进行的比较实验在同时检测AFM1和MEL在牛奶中发现了很好的一致性。这种提出的方​​法可以广泛用于同时监测多级化学污染物，以确保食品安全。

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  • 来源
    《Analytical chemistry》 |2019年第3期|共6页
  • 作者

    Li Guanghua; Xu Lin; Wu Wenqin; Wang Du; Jiang Jun; Chen Xiaomei; Zhang Wen; Poapolathep Saranya; Poapolathep Amnart; Zhang Zhaowei; Zhang Qi; Li Peiwu;

  • 作者单位

    Chinese Acad Agr Sci Oil Crops Res Inst Wuhan 430062 Hubei Peoples R China;

    Chinese Acad Agr Sci Oil Crops Res Inst Wuhan 430062 Hubei Peoples R China;

    Chinese Acad Agr Sci Oil Crops Res Inst Wuhan 430062 Hubei Peoples R China;

    Chinese Acad Agr Sci Oil Crops Res Inst Wuhan 430062 Hubei Peoples R China;

    Chinese Acad Agr Sci Oil Crops Res Inst Wuhan 430062 Hubei Peoples R China;

    Chinese Acad Agr Sci Oil Crops Res Inst Wuhan 430062 Hubei Peoples R China;

    Chinese Acad Agr Sci Oil Crops Res Inst Wuhan 430062 Hubei Peoples R China;

    Kasetsart Univ Fac Vet Med Dept Pharmacol Bangkok 10900 Thailand;

    Kasetsart Univ Fac Vet Med Dept Pharmacol Bangkok 10900 Thailand;

    Chinese Acad Agr Sci Oil Crops Res Inst Wuhan 430062 Hubei Peoples R China;

    Minist Agr Key Lab Biol &

    Genet Improvement Oil Crops Wuhan 430062 Hubei Peoples R China;

    Minist Agr Key Lab Biol &

    Genet Improvement Oil Crops Wuhan 430062 Hubei Peoples R China;

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  • 正文语种 eng
  • 中图分类 分析化学;
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