...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Analytical chemistry >Nanoparticle Analysis by Online Comprehensive Two-Dimensional Liquid Chromatography combining Hydrodynamic Chromatography and Size-Exclusion Chromatography with Intermediate Sample Transformation
【24h】

Nanoparticle Analysis by Online Comprehensive Two-Dimensional Liquid Chromatography combining Hydrodynamic Chromatography and Size-Exclusion Chromatography with Intermediate Sample Transformation

机译：通过在线综合二维液相色谱法分析水动力色谱和尺寸排阻色谱与中间样品转化的纳米粒子分析

获取原文
获取原文并翻译 | 示例
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Polymeric nanoparticles have become indispensable in modern society with a wide array of applications ranging from waterborne coatings to drug-carrier-delivery systems. While a large range of techniques exist to determine a multitude of properties of these particles, relating physicochemical properties of the particle to the chemical structure of the intrinsic polymers is still challenging. A novel, highly orthogonal separation system based on comprehensive two-dimensional liquid chromatography (LC x LC) has been developed. The system combines hydrodynamic chromatography (HDC) in the first-dimension to separate the particles based on their size, with ultrahigh-performance size-exclusion chromatography (SEC) in the second dimension to separate the constituting polymer molecules according to their hydrodynamic radius for each of 80 to 100 separated fractions. A chip-based mixer is incorporated to transform the sample by dissolving the separated nanoparticles from the first-dimension online in tetrahydrofuran. The polymer bands are then focused using stationary-phase-assisted modulation to enhance sensitivity, and the water from the first-dimension eluent is largely eliminated to allow interaction-free SEC. Using the developed system, the combined two-dimensional distribution of the particle-size and the molecular-size of a mixture of various polystyrene (PS) and polyacrylate (PACR) nanoparticles has been obtained within 60 min.
机译：聚合物纳米颗粒在现代社会中是不可或缺的，其中各种应用范围从水性涂料到药物 - 载体输送系统。虽然存在大范围的技术来确定这些颗粒的多种性质，但将颗粒的物理化学性质与本征聚合物的化学结构相关，仍然是挑战性的。已经开发了一种基于综合二维液相色谱（LC X LC）的新型高度正交分离系统。该系统将第一尺寸中的流体动力色谱（HDC）与基于其尺寸的尺寸与第二尺寸中的超高性能尺寸排阻色谱（SEC）相结合，以将构成聚合物分子分离为每个尺寸80至100分分数。掺入基于芯片的混合器以通过将分离的纳米颗粒从在鼠中在四氢呋喃中的第一维度溶解来转化样品。然后使用静止相位辅助调制聚焦聚合物带以增强灵敏度，并且来自第一维洗脱液的水很大程度上消除以允许无间隙的秒。使用开发系统，在60分钟内获得了各种聚苯乙烯（PS）和聚丙烯酸酯（PACR）和聚丙烯酸酯（PACR）纳米颗粒的混合物的组合二维分布和分子尺寸。

著录项

  • 来源
    《Analytical chemistry》 |2017年第17期|共8页
  • 作者

    Pirok Bob W. J.; Abdulhussain Noor; Aalbers Tom; Wouters Bert; Peters Ron A. H.; Schoenmakers Peter J.;

  • 作者单位

    Univ Amsterdam Analyt Chem Grp vant Hoff Inst Mol Sci Sci Pk 904 NL-1098 XH Amsterdam Netherlands;

    Univ Amsterdam Analyt Chem Grp vant Hoff Inst Mol Sci Sci Pk 904 NL-1098 XH Amsterdam Netherlands;

    Univ Amsterdam Analyt Chem Grp vant Hoff Inst Mol Sci Sci Pk 904 NL-1098 XH Amsterdam Netherlands;

    Univ Amsterdam Analyt Chem Grp vant Hoff Inst Mol Sci Sci Pk 904 NL-1098 XH Amsterdam Netherlands;

    Univ Amsterdam Analyt Chem Grp vant Hoff Inst Mol Sci Sci Pk 904 NL-1098 XH Amsterdam Netherlands;

    Univ Amsterdam Analyt Chem Grp vant Hoff Inst Mol Sci Sci Pk 904 NL-1098 XH Amsterdam Netherlands;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 分析化学;
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号