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首页> 外文期刊>Analytica chimica acta >Duplex-specific nuclease assisted miRNA assay based on gold and silver nanoparticles co-decorated on electrode interface
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Duplex-specific nuclease assisted miRNA assay based on gold and silver nanoparticles co-decorated on electrode interface

机译：基于金和银纳米颗粒在电极界面上装饰的金和银纳米颗粒的双链核核酸酶辅助miRNA测定

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摘要

miRNAs are small non-coding RNAs for gene regulation, which serve as promising biomarkers for the diagnosis of certain diseases. In this contribution, we have proposed a convenient electrochemical biosensing strategy based on the interaction between DNA modified gold nanoparticles (AuNPs) and silver nanoparticles (AgNPs). In principle, citrate capped AuNPs and AgNPs can be co-decorated on the electrode successively. However, with the modification of DNA on AuNPs surface, a strong negative layer is formed. AuNPs@DNA modified electrode could then inhibit subsequent adsorption of AgNPs due to the electrostatic repulsion and steric hindrance effect. As a result, electrochemical response from AgNPs is significantly decreased. On the other hand, in the presence of target miRNA, DNA on AuNPs hybridizes with miRNA and can thus be cyclically digested by duplex-specific nuclease (DSN). Without the shield of DNA, AgNPs can be relaunched at the AuNPs modified electrode. By analyzing the silver stripping peak, highly sensitive detection of miRNA can be achieved. This biosensor exhibits the limit of detection as low as 0.62 fM and a broad linear range from 1 fM to 1 pM. It may hold great potential utility for miRNA assay in the applications of biomedical researches and early clinical diagnosis. (C) 2020 Elsevier B.V. All rights reserved.
机译：miRNA是小型非编码RNA用于基因调控，其作为有前途的生物标志物，用于诊断某些疾病。在这一贡献中，我们提出了一种基于DNA改性金纳米颗粒（AUNP）和银纳米颗粒（AgNP）之间的相互作用的方便的电化学生物腐蚀策略。原则上，柠檬酸覆盖的AUNP和AGNP可以连续地在电极上装饰。然而，随着DNA在AUNPS表面上的修饰，形成强负层。然后，AUNPS @ DNA改性电极可以由于静电排斥和空间阻断效应而抑制随后的AgNP吸附。结果，来自AGNP的电化学反应显着降低。另一方面，在靶miRNA存在下，肛肠膜的DNA用miRNA杂交，因此可以通过双相特异性核酸酶（DSN）循环消化。如果没有DNA的屏蔽，AgNP可以在AUNPS改性电极上重新抓住。通过分析银汽提峰，可以实现高敏感的miRNA检测。这种生物传感器显示出低至0.62 fm的检测限，并且宽线性范围为1 fm至1pm。它可能在生物医学研究和早期临床诊断中占MIRNA测定的巨大潜在效用。 （c）2020 Elsevier B.V.保留所有权利。

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