...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Analytica chimica acta >On-column and gradient focusing-induced high-resolution separation in narrow open tubular liquid chromatography and a simple and economic approach for pico-gradient separation
【24h】

On-column and gradient focusing-induced high-resolution separation in narrow open tubular liquid chromatography and a simple and economic approach for pico-gradient separation

机译：柱上和梯度聚焦诱导的高分辨率分离在窄的露天管状液相色谱中，以及微微梯度分离的简单和经济方法

获取原文
获取原文并翻译 | 示例
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We have recently obtained extraordinarily high efficiencies and sharp peaks using narrow open tubular (OT) columns for liquid chromatographic separations. On-column focusing is commonly observed in liquid chromatography, but this effect alone could not satisfactorily explain the sharpness of these peaks. In this work we investigated the reasons that could have led to the peak sharpness. We hypothesized initially that analytes confined in a narrow OT column might have significantly reduced analyte diffusivities and the reduced diffusivities consequently resulted in the peak sharpness. This hypothesis was invalidated immediately after we measured the diffusion coefficients and did not notice any noticeable diffusivity increases of the analytes inside such columns. We then designed an experiment and revealed a "re-focusing effect". Investigation of this re-focusing effect eventually led us to the observation of a gradient focusing caused by the composition difference between the eluent and the sample matrix. It was this gradient focusing that had contributed primarily to the peak sharpness. On the basis of this insightful understanding, we further developed a simple and economic approach to perform pico-gradient narrow open tubular liquid chromatographic separations. (C) 2019 Elsevier B.V. All rights reserved.
机译：我们最近使用窄的张开管（OT）柱进行了非常高的效率和锋利的峰值，用于液相色谱分离。在液相色谱中通常观察到柱子聚焦，但单独的这种效果不能令人满意地解释这些峰的锐度。在这项工作中，我们调查了可能导致锐利尖锐度的原因。首先，我们初步假设狭窄在窄耳中的分析物可能具有显着降低的分析物扩散性，并且因此导致降低的扩散性导致尖峰清晰度。在测量扩散系数之后，该假设立即失效，并且没有注意到这种柱内的分析物的任何明显的扩散性增加。然后我们设计了一个实验并揭示了“重新聚焦效果”。对这种重聚焦效应的研究最终导致我们观察由洗脱液和样品基质之间的成分差异引起的梯度聚焦。这是这种渐变聚焦，主要是良好的锐利。在这种洞察力的基础上，我们进一步开发了一种简单而经济的方法，可以进行微微梯度窄的露天管状液相色谱分离。 （c）2019年Elsevier B.V.保留所有权利。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号