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首页> 外文期刊>Analytical and bioanalytical chemistry >A liquid chromatography method using a monolithic column for the determination of corticoids in animal feed and animal feeding water
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A liquid chromatography method using a monolithic column for the determination of corticoids in animal feed and animal feeding water

机译：一种使用整体柱用于测定动物饲料和动物饲养水中皮质醇的液相色谱法

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摘要

An HPLC-DAD method for determining corticoids in calf feed and in animal feeding water samples using a monolithic column has been developed and validated. The method optimization included the study of binary mobile phases of water and acetonitrile. The optimum separation was achieved at 40 degrees C, with acetonitrile:H2O 29:71 v/v used as mobile phase and a 3 ml/min flow-rate, which resulted in their separation in about 5 min. Two reported sample procedures were applied to feed and for animal feeding water samples prior to HPLC. Method validation was carried out according to the EU criteria established for quantitative screening methods. The results indicate that this method is highly specific, reproducible and accurate. The proposed method was found to be robust and unaffected by small variations in the extraction procedure and in HPLC conditions. The developed method for the determination of corticoids in feed and water samples was also found to be suitable for different kinds of feeds and waters.
机译：已经开发出并验证了用于使用整体柱测定小牛饲料中皮质醇的HPLC-DAD方法，并使用整体柱验证。该方法优化包括水和乙腈的二元流动相的研究。在40℃下实现最佳分离，用乙腈：H 2 O 29:71 V / V作为流动相和3ml / min流速，导致其在约5分钟内分离。在HPLC之前应用了两种报道的样品程序以进料和用于动物喂水水样。方法验证根据为定量筛查方法建立的欧盟标准进行。结果表明，该方法具有高度特异性，可重复和准确的。发现所提出的方法是鲁棒，不受萃取过程和HPLC条件下的小变异的影响。还发现，用于测定饲料和水样中皮质醇的方法适用于不同种类的饲料和水。

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