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Changes in microbial community in the presence of oil and chemical dispersant and their effects on the corrosion of API 5L steel coupons in a marine-simulated microcosm

机译:在油化学分散剂存在下微生物群落的变化及其对海洋模拟微观循粒体中API 5L钢杯腐蚀的影响

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The influence of crude oil and chemical dispersant was evaluated over planktonic bacteria and biofilms grown on API 5L steel surfaces in microcosm systems. Three conditions were simulated, an untreated marine environment and a marine environment with the presence of crude oil and a containing crude oil and chemical dispersant. The results of coupon corrosion rates indicated that in the oil microcosm, there was a high corrosion rate when compared with the other two systems. Analysis of bacterial communities by 16S rRNA gene sequencing described a clear difference between the different treatments. In plankton communities, theBacilliandGammaproteobacteriaclasses were the most present in numbers of operational taxonomic unit (OTUs). TheVibrionales,Oceanospirillales, andAlteromonadalesorders were predominant in the treatment with crude oil, whereas in the microcosm containing oil and chemical dispersant, mainly members ofBacillalesorder were detected. In the communities analyzed from biofilms attached to the coupons, the most preponderant class wasAlphaproteobacteria, followed byGammaproteobacteria. In the control microcosm, there was a prevalence of the ordersRhodobacterales,Aeromonadales, andAlteromonadales, whereas in the dispersed oil and oil systems, the members of the orderRhodobacteraleswere present in a larger number of OTUs. These results demonstrate how the presence of a chemical dispersant and oil influence the corrosion rate and bacterial community structures present in the water column and biofilms grown on API 5L steel surfaces in a marine environment.
机译:在微观体系中的API 5L钢表面上生长的浮鳞菌和生物膜评估了原油和化学分散剂的影响。模拟了三种条件,未经处理的海洋环境和海洋环境,存在原油和含有原油和化学分散剂的原油。优惠券腐蚀速率的结果表明,在油微科,与其他两个系统相比,腐蚀速率很高。通过16S rRNA基因测序分析细菌群组的分析描述了不同治疗之间的明显差异。在Plankton社区中,碧桂蛋白犬基曲霉杆菌是最多存在的运作分类单位(OTUS)。 TheVibrionales,Oceanomirillales,AndalteroMonadalesorders在用原油处理中占主导地位,而在含有油和化学分散剂的微观粒子中,主要检测到伯氏霉素的成员。在从附着于优惠券的生物膜分析的社区中,最优先级患者患者,其次是Makamprooteobacteria。在对照微观体中,患有OrdersRhocococobacterales,Aeromonadales,AndalteroMonadales的患病率,而在分散的油和油系统中,ourtrhodobocateralesswere的成员存在于更多的Otus中。这些结果表明了化学分散剂和油的存在如何影响水柱和在海洋环境中在API 5L钢表面上生长的水柱和生物膜中存在的腐蚀速率和细菌群落结构。

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