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首页> 外文期刊>Applied Microbiology and Biotechnology >Re-engineering Escherichia coli KJ122 to enhance the utilization of xylose and xylose/glucose mixture for efficient succinate production in mineral salt medium
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Re-engineering Escherichia coli KJ122 to enhance the utilization of xylose and xylose/glucose mixture for efficient succinate production in mineral salt medium

机译：重新设计大肠杆菌KJ122以增强木糖和木糖/葡萄糖混合物的利用，以便在矿物盐培养基中高效琥珀酸盐生产

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摘要

Escherichia coli KJ122 was previously engineered to produce high concentration and yield of succinate in mineral salt medium containing glucose and sucrose under anaerobic conditions. However, this strain does not efficiently utilize xylose. To improve the xylose uptake and utilization in the strain KJ122, xylFGH and xylE genes were individually and simultaneously deleted. E. coli KJ12201 (KJ122::Delta xylFGH) exhibited superior abilities in growth, xylose consumption, and succinate production compared to those of the parental strain KJ122. However, E. coli KJ12202 (KJ122::Delta xylE) lessened xylose consumption due to an ATP deficit for metabolizing xylose thus making succinate production from xylose not preferable. Moreover, E. coli KJ12203 (KJ122::Delta xylFGH Delta xylE) exhibited an impaired growth on xylose due to lacking of xylose transporters. After performing metabolic evolution, the evolved KJ12201-14T strain exhibited a great improvement in succinate production from pure xylose with higher concentration and productivity about 18 and 21%, respectively, compared to KJ12201 strain. During fed-batch fermentation, KJ12201-14T also produced succinate from xylose at a concentration, yield, and overall productivity of 84.6 +/- 0.7 g/L, 0.86 +/- 0.01 g/g and 1.01 +/- 0.01 g/L/h, respectively. KJ12201 and KJ12201-14T strains co-utilized glucose/xylose mixture without catabolite repression. Both strains produced succinate from glucose/xylose mixture at concentration, yield, and overall and specific productivities of about 85 g/L, 0.85 g/g, 0.70 g/L/h, and 0.44 g/gCDW/h, respectively. Based on our results, KJ12201 and KJ12201-14T strains exhibited a greater performance in succinate production from xylose containing medium than those of other published works. They would be potential strains for the economic bio-based succinate production from xylose.
机译：Escherichia Coli KJ122先前经过设计，以在厌氧条件下含有葡萄糖和蔗糖的矿物盐培养基中产生高浓度和产率。然而，这种菌株没有有效地利用木糖。为了改善菌株KJ122中的木糖摄取和利用，单独删除Xylfgh和Xyle基因。与亲本菌株KJ122相比，大肠杆菌KJ12201（kJ122 :: delta xylfgh）表现出具有优异的生长，木糖消耗和琥珀酸生产的能力。然而，大肠杆菌KJ12202（KJ122 :: Delta Xyle）由于ATP缺口而降低了木糖消耗，用于代谢木糖，从而使琥珀酸盐从木糖产生不优选。此外，大肠杆菌KJ12203（KJ122 :: Delta Xylfgh DeltaXyle）由于缺乏木糖转运蛋白，在木糖上表现出损伤的生长。在进行代谢进化之后，进化的KJ12201-14T菌株在与KJ12201菌株相比，分别具有较高浓度和生产率的琥珀酸盐产生的琥珀酸盐产生的巨大改善。在喂食批量发酵期间，KJ12201-14T也以浓度，产率和总生产率产生84.6 +/- 0.7g / L，0.86 +/- 0.01g / g和1.01 +/- 0.01g / L期间的琥珀酸盐。 / h分别。 KJ12201和KJ12201-14T应菌株共用葡萄糖/木糖混合物，没有抗粘合剂抑制。两种菌株分别以浓度，产率和总体和特异性产品的浓度，产率和总产值产生约85g / L，0.85g / g，0.70g / L / h和0.44g / gcdw / h的琥珀酸盐。基于我们的结果，KJ12201和KJ12201-14T菌株在含有含有其他公布作品的含培养基中的琥珀酸酯生产中表现出更大的性能。它们将是由木糖的经济生物生物琥珀酸盐生产的潜在菌株。

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