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首页> 外文期刊>Applied Microbiology and Biotechnology >Differential effect of early antibiotic intervention on bacterial fermentation patterns and mucosal gene expression in the colon of pigs under diets with different protein levels
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Differential effect of early antibiotic intervention on bacterial fermentation patterns and mucosal gene expression in the colon of pigs under diets with different protein levels

机译：早期抗生素干预对不同蛋白质水平的饮食中猪结肠癌细菌发酵模式和粘膜基因表达的差异

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摘要

The study aimed to evaluate the effects of early antibiotic intervention (EAI) on bacterial fermentation patterns and mucosal immune markers in the colon of pigs with different protein level diets. Eighteen litters of piglets at day (d) 7 were fed creep feed without or with growth promoting antibiotics until d 42. At d 42, pigs within each group were further randomly assigned to a normal- or low-crude protein (CP) diet. At d 77 and d 120, five pigs per group were slaughtered for analyzing colonic bacteria, metabolites, and mucosal gene expressions. Results showed that low-CP diet increased propionate and butyrate concentrations at d 77 but reduced ammonia and phenol concentrations (P < 0.05). EAI increased p-cresol and indole concentrations under normal-CP diet at d 77 (P < 0.05). Low-CP diet significantly affected (P < 0.05) some bacteria groups (Firmicutes, Clostridium cluster IV, Clostridium cluster XIVa, Escherichia coli, and Lactobacillus), but EAI showed limited effects. Low-CP diet down-regulated gene expressions of pro-inflammatory cytokines, toll-like receptor (TLR4), myeloid differentiating factor 88 (MyD88), and nuclear factor-kappa B p65 (NF-kappa B p65) (P < 0.05). EAI up-regulated mRNA expressions of interleukin-8 (IL-8) and interferon-gamma (IFN-gamma) under normal-CP diet at d 77 (P < 0.05). Furthermore, reductions of E. coli and ammonia under low-CP diet were positively correlated with down-regulated gene expressions of pro-inflammatory cytokines, which were positively correlated with the down-regulated TLR4-MyD88-NF-kappa B signaling pathway. In conclusion, EAI had short-term effects under normal-CP diet with increased aromatic amino acid fermentation and gene expressions of pro-inflammatory cytokines. Low-CP diet markedly reduced protein fermentation, modified microbial communities, and down-regulated gene expressions of pro-inflammatory cytokines possibly via down-regulating TLR4-MyD88-NF-kappa B signaling pathway.
机译：该研究旨在评估早期抗生素干预（EAI）对具有不同蛋白质水平饮食的猪结肠癌细菌发酵模式和粘膜免疫标志物的影响。 Day（D）7的18只仔猪喂养蠕变饲料没有或增长促进抗生素直至D 42.在D 42中，每组内的猪进一步随机分配给正常或低粗蛋白（CP）饮食。在D 77和D 120中，屠宰5只猪以分析结肠细菌，代谢物和粘膜基因表达。结果表明，低CP饮食在D 77的丙酸盐和丁酸丁浓度上增加，但氨和苯酚浓度降低（P <0.05）。 EAI在D 77的正常CP饮食下增加p-甲酚和吲哚浓度（P <0.05）。低CP饮食显着影响（P <0.05）一些细菌群（陈旧，梭菌群，梭菌群Xiva，大肠杆菌和乳酸杆菌），但EAI效果有限。低CP饮食下调基因表达的促炎细胞因子，可收费受体（TLR4），髓样区分因子88（MYD88）和核因子-Kappa B p65（NF-Kappa B p65）（P <0.05） 。在D 77的正常-CP饮食下，EAI上调的白细胞介素-8（IL-8）和干扰素-γ（IFN-Gamma）的升高的mRNA表达（P <0.05）。此外，低CP饮食下的大肠杆菌和氨的减少与促炎细胞因子的下调基因表达呈正相关，其与下调的TLR4-MYD88-NF-Kappa发信号通路呈正相关。总之，EAI在正常-CP饮食下具有短期影响，具有增加的芳族氨基酸发酵和促炎细胞因子的基因表达。低CP饮食可能通过下调TLR4-MYD88-NF-Kappa B信号通路，显着降低蛋白质发酵，修饰的微生物群落和促炎细胞因子的下调基因表达。

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  • 来源
  • 作者

    Zhang Chuanjian; Yu Miao; Yang Yuxiang; Mu Chunlong; Su Yong; Zhu Weiyun;

  • 作者单位

    Nanjing Agr Univ Coll Anim Sci &

    Technol Jiangsu Key Lab Gastrointestinal Nutr &

    Anim Hlth Lab Gastrointestinal Microbiol Nanjing 210095 Jiangsu Peoples R China;

    Nanjing Agr Univ Coll Anim Sci &

    Technol Jiangsu Key Lab Gastrointestinal Nutr &

    Anim Hlth Lab Gastrointestinal Microbiol Nanjing 210095 Jiangsu Peoples R China;

    Nanjing Agr Univ Coll Anim Sci &

    Technol Jiangsu Key Lab Gastrointestinal Nutr &

    Anim Hlth Lab Gastrointestinal Microbiol Nanjing 210095 Jiangsu Peoples R China;

    Nanjing Agr Univ Coll Anim Sci &

    Technol Jiangsu Key Lab Gastrointestinal Nutr &

    Anim Hlth Lab Gastrointestinal Microbiol Nanjing 210095 Jiangsu Peoples R China;

    Nanjing Agr Univ Coll Anim Sci &

    Technol Jiangsu Key Lab Gastrointestinal Nutr &

    Anim Hlth Lab Gastrointestinal Microbiol Nanjing 210095 Jiangsu Peoples R China;

    Nanjing Agr Univ Coll Anim Sci &

    Technol Jiangsu Key Lab Gastrointestinal Nutr &

    Anim Hlth Lab Gastrointestinal Microbiol Nanjing 210095 Jiangsu Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 应用微生物学;生物工程学（生物技术）;
  • 关键词

    Antibiotic; Low-crude protein diet; Metabolites; Microbiota; Gene expression; Pigs;

    机译：抗生素;低粗蛋白质饮食;代谢物;微生物群;基因表达;猪;

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