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首页> 外文期刊>Applied Microbiology and Biotechnology >Production of optically pure D-lactic acid from brown rice using metabolically engineered Lactobacillus plantarum
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Production of optically pure D-lactic acid from brown rice using metabolically engineered Lactobacillus plantarum

机译：使用代谢工程乳酸盐植物的糙米生产来自糙米的光学纯D-乳酸

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摘要

Simultaneous saccharification and fermentation (SSF) of d-lactic acid was performed using brown rice as both a substrate and a nutrient source. An engineered Lactobacillus plantarum NCIMB 8826 strain, in which the EY-lactate dehydrogenase gene was disrupted, produced 97.7 g/L d-lactic acid from 20% (w/v) brown rice without any nutrient supplementation. However, a significant amount of glucose remained unconsumed and the yield of lactic acid was as low as 0.75 (g/g-glucose contained in brown rice). Interestingly, the glucose consumption was significantly improved by adapting L. plantarum cells to the low-pH condition during the early stage of SSF (8-17 h). As a result, 117.1 g/L d-lactic acid was produced with a high yield of 0.93 and an optical purity of 99.6% after 144 h of fermentation. SSF experiments were repeatedly performed for ten times and d-lactic acid was stably produced using recycled cells (118.4-129.8 g/L). On average, d-lactic acid was produced with a volumetric productivity of 2.18 g/L/h over 48 h.
机译：使用糙米作为基材和营养源的糙米进行D-乳酸的同时糖化和发酵（SSF）。工程化乳杆菌植物NCIMB 8826菌株，其中破坏了Ey乳酸脱氢酶基因，从20％（w / v）糙米中产生97.7g / l d-乳酸，没有任何营养素的补充。然而，大量的葡萄糖仍然不少，乳酸的产率低至0.75（糙米中含有的G / G-葡萄糖）。有趣的是，通过在SSF的早期阶段（8-17小时）在低pH病症中，通过适应L.Plastarum细胞来显着改善葡萄糖消耗。结果，在144小时后，在发酵后，高产率为0.93的高产率为0.93的光学纯度为117.1g / l d-乳酸。重复进行SSF实验，使用再循环细胞（118.4-129.8g / l）稳定地制备D-乳酸。平均而言，产生D-乳酸，体积生产率为2.18g / l / h超过48小时。

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