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首页> 外文期刊>Applied Microbiology and Biotechnology >Beyond bread and beer: whole cell protein extracts from baker's yeast as a bulk source for 3D cell culture matrices
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Beyond bread and beer: whole cell protein extracts from baker's yeast as a bulk source for 3D cell culture matrices

机译：超越面包和啤酒：全细胞蛋白质从面包酵母酵母提取物作为3D细胞培养基质的散装来源

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摘要

Here, we present a novel approach to form hydrogels from yeast whole cell protein. Countless hydrogels are available for sophisticated research, but their fabrication is often difficult to reproduce, with the gels being complicated to handle or simply too expensive. The yeast hydrogels presented here are polymerized using a four-armed, amine reactive crosslinker and show a high chemical and thermal resistance. The free water content was determined by measuring swelling ratios for different protein concentrations, and in a freeze-drying approach, pore sizes of up to 100 mu m in the gel could be created without destabilizing the 3D network. Elasticity was proofed to be adjustable with the help of atomic force microscopy by merely changing the amount of used protein. Furthermore, the material was tested for possible cell culture applications; diffusion rates in the network are high enough for sufficient supply of human breast cancer cells and adenocarcinomic human alveolar basal epithelial cells with nutrition, and cells showed high viabilities when tested for compatibility with the material. Furthermore, hydrogels could be functionalized with RGD peptide and the optimal concentration for sufficient cell adhesion was determined to be 150 mu M. Given that yeast protein is one of the cheapest and easiest available protein sources and that hydrogels are extremely easy to handle, the developed material has highly promising potential for both sophisticated cell culture techniques as well as for larger scale industrial applications.
机译：在这里，我们提出了一种新的方法来形成来自酵母全细胞蛋白的水凝胶。无数水凝胶可用于复杂的研究，但它们的制作往往难以繁殖，凝胶复杂地处理或者太昂贵。这里呈现的酵母水凝胶使用四臂，胺反应性交联剂聚合并显示出高化学和热阻。通过测量不同蛋白质浓度的溶胀比测定自由含水量，并且在冷冻干燥方法中，可以在不打击3D网络的情况下产生凝胶中高达100μm的孔径。通过仅改变二手蛋白质的量，可以通过原子力显微镜的帮助可调节弹性。此外，测试该材料以进行可能的细胞培养应用;网络中的扩散速率足够高，足以提供足够的人乳腺癌细胞和腺癌人肺泡基底上皮细胞，并且细胞在测试与材料的相容性时显示出高的活力。此外，水凝胶可以用RGD肽官能化，并且测定足够的细胞粘附的最佳浓度为150μm。鉴于酵母蛋白是最便宜和最简单的蛋白质来源，并且水凝胶极易处理，所发达的材料对复杂的细胞培养技术以及更大尺度的工业应用具有高度有希望的潜力。

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