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首页> 外文期刊>Applied Microbiology and Biotechnology >Identification of alkaline phosphatase genes for utilizing a flame retardant, tris(2-chloroethyl) phosphate, in Sphingobium sp strain TCM1
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Identification of alkaline phosphatase genes for utilizing a flame retardant, tris(2-chloroethyl) phosphate, in Sphingobium sp strain TCM1

机译：用于利用阻燃剂的碱性磷酸酶基因的碱性磷酸酶基因，Sphinguium SP菌株TCM1中的阻燃剂（2-氯乙基）磷酸盐

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摘要

Tris(2-chloroethyl) phosphate (TCEP) is a haloalkyl phosphate flame retardant and plasticizer that has been recognized as a global environmental contaminant. Sphingobium sp. strain TCM1 can utilize TCEP as a phosphorus source. To identify the phosphomonoesterase involved in TCEP utilization, we identified four putative alkaline phosphatase (APase) genes, named SbphoA, SbphoD1, SbphoD2, and SbphoX-II, in the genome sequence. Following expression of these genes in Escherichia coli, APase activity was confirmed for the SbphoA and SbphoX-II gene products but was not clearly observed for the SbphoD1 and SbphoD2 gene products, owing to their accumulation in inclusion bodies. The single deletion of either SbphoA or SbphoX-II retarded the growth and reduced the APase activity of strain TCM1 cells on medium containing TCEP as the sole phosphorus source; these changes were more marked in cells with the SbphoX-II gene deletion. In contrast, the deletion of either SbphoD1 or SbphoD2 had no effect on cell growth or APase activity. The double deletion of SbphoA and SbphoX-II resulted in the complete loss of cell growth on TCEP. These results show that SbPhoA and SbPhoX-II are involved in the utilization of TCEP as a phosphorus source and that SbPhoX-II is the major phosphomonoesterase involved in TCEP utilization.
机译：三（2-氯乙基）磷酸盐（TCEP）是磷酸卤烷基阻燃剂和增塑剂，其已被认为是全球环境污染物。鞘豆植物sp。菌株TCM1可以利用TCEP作为磷来源。为了鉴定参与TCEP利用的磷酸酯酶，在基因组序列中鉴定了四种推定的碱性磷酸酶（APASE）基因，名为SBPHOA，SBPHOD1，SBPHOD2和SBPHOX-II。在大肠杆菌中表达这些基因后，对于Sbphox和Sbphox-II基因产物证实了Apase活性，但由于它们在包涵体中的积累而没有清楚地观察到Sbphod1和Sbphod2基因产物。 Sbphoa或Sbphox-II的单一缺失延迟了生长并降低了含有TCEP作为唯一磷来源的培养基菌株TCM1细胞的Apase活性;这些变化在细胞中更具标记，具有Sbphox-II基因缺失。相反，Sbphod1或Sbphod2的缺失对细胞生长或嗜睡活性没有影响。双缺失的Sbphoa和Sbphox-II导致TCEP上的细胞生长完全丧失。这些结果表明，Sbphoa和Sbphox-II参与了TCEP作为磷来源的利用，Sbphox-II是参与TCEP利用率的主要磷酸酯酶。

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