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首页> 外文期刊>Applied Microbiology and Biotechnology >Mutation on Gly115 and Tyr205 of the cyclic dipeptide C2-prenyltransferase FtmPT1 increases its catalytic activity toward hydroxynaphthalenes
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Mutation on Gly115 and Tyr205 of the cyclic dipeptide C2-prenyltransferase FtmPT1 increases its catalytic activity toward hydroxynaphthalenes

机译：环二肽C2-戊烯转移酶FTMPT1的GLY115和TYR205的突变增加了其催化活性朝向羟基萘

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摘要

The fungal cyclic dipeptide prenyltransferase FtmPT1 from Aspergillus fumigatus catalyzes a regular C2-prenylation of brevianamide F (cyclo-l-Trp-l-Pro) and is involved in the biosynthesis of a number of biologically active natural products including tryprostatins, spirotryprostatins, verruculogen, and fumitremorgins. FtmPT1, like other members of the dimethylallyltryptophan synthase superfamily, was shown to have high substrate promiscuity for tryptophan-containing cyclic dipeptides and a few other aromatic substrates. A previous study demonstrated the acceptance of 1-naphthol by FtmPT1, but with very low product yield. In this study, we report the significantly increased acceptance of 1-naphthol and other hydroxynaphthalenes by FtmPT1_G115A and six FtmPT1_Y205X single mutants as well as FtmPT1_G115A_Y205C. These results provided an example for creation of biocatalysts with improved catalytic activity by site-directed mutagenesis.
机译：真菌环状二肽prenyl转移酶Ftmpt1来自曲霉属fumigatus催化Brevianamide f（Cyclo-L-TRP-L-Pro）的常规C2-戊基化，并且参与了许多生物活性天然产物的生物合成，包括试验抑制蛋白，螺霉素，疣状， 和fumitemorgins。 FTMPT1，如二甲基嗜含量合成酶超家族的其他成员，显示出含色氨酸的环状二肽和少数其他芳族基材的高底物滥交。 以前的研究证明了FTMPT1的接受1-萘酚，但产品产量非常低。 在这项研究中，我们通过FTMPT1_G115A和六种FTMPT1_Y205X单突变体以及FTMPT1_G115A_Y205C报告了1-萘酚和其他羟基萘的接受显着增加。 这些结果提供了通过定向定向诱变产生具有改善的催化活性的生物催化剂的示例。

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