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首页> 外文期刊>Applied Microbiology and Biotechnology >Screening for proteolytically active lactic acid bacteria and bioactivity of peptide hydrolysates obtained with selected strains
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Screening for proteolytically active lactic acid bacteria and bioactivity of peptide hydrolysates obtained with selected strains

机译：用选定菌株获得的蛋白水解活性乳酸菌和肽水解产物的生物活性筛选

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摘要

In a screening for proteolytically active lactic acid bacteria, three strains, Lactobacillus delbrueckii ssp. lactis 92202, Lactobacillus helveticus 92201, and Lactobacillus delbrueckii ssp. bulgaricus 92059, showed the highest activities following growth in milk. All three strains degraded alpha- and beta-casein, but did not hydrolyse kappa-casein. HPLC analysis of skim milk fermentation revealed increasing amounts of peptides after 5 and 10 h with Lb. d. ssp. bulgaricus 92059. Hydrolysates obtained with Lb. d. ssp. lactis 92202 and Lb. d. ssp. bulgaricus 92059 revealed the highest angiotensin-converting enzyme-inhibitory effect. The effect was dose dependent. Almost no effect (< 10%) was seen for Lb. helveticus 92201. For Lb. d. ssp. bulgaricus 92059, maximal inhibition of approx. 65% was reached after 25 h of fermentation. In an in vitro assay measuring potential immunomodulation, hydrolysates of the three strains yielded anti-inflammatory activities in the presence of TNF-alpha. However, the effects were more pronounced at lower hydrolysate concentrations. In the absence of TNF-alpha, slight pro-inflammatory effects were observed. The hydrolysate of Lb. d. ssp. bulgaricus 92059, when purified by means of solid-phase extraction, exhibited pro-inflammatory activity. Sour whey containing Lb. d. ssp. bulgaricus 92059 cells showed pro-inflammatory activity while cell-free sour whey was clearly anti-inflammatory. In the purified hydrolysate, 20 different alpha- and beta-casein (CN)derived peptides could be identified by LC-MS. Most peptides originated from the central and C-terminal regions of beta-casein. Peptide length was between 9 (beta-CN(f 59-67)) and 22 amino acids (beta-CN(f 117-138)).
机译：在蛋白水解活性乳酸菌的筛选中，三种菌株，Lactobacillus delbrueckii ssp。 Lactis 92202，乳杆菌Helveticus 92201和Lactobacillus delbrueckii SSP。保加利宫92059，牛奶生长后展示了最高的活动。所有三种菌株都降解了α-和β-酪蛋白，但没有水解Kappa-酪蛋白。脱脂乳发酵的HPLC分析显示，含量为5至10小时后的肽量增加。天。 SSP。保加利亚宫92059.用LB获得的水解产物。天。 SSP。 Lactis 92202和LB.天。 SSP。保加利宫92059揭示了最高的血管紧张素转换酶抑制作用。效果是剂量依赖性的。对于LB而言，几乎没有效果（<10％） Helveticus 92201.对于LB.天。 SSP。保加利宫92059，最大抑制约。发酵25小时后达到65％。在体外测定测量潜在免疫调节中，三种菌株的水解产物在TNF-α存在下产生抗炎活性。然而，在较低水解的浓度下，效果更加明显。在没有TNF-α的情况下，观察到轻微的促炎作用。 LB的水解产物。天。 SSP。通过固相萃取纯化时，保加利亚病92059表现出促炎活性。酸奶乳清含有LB.天。 SSP。保加利宫92059细胞显示出促炎活性，而无细胞酸乳清清楚地抗炎。在纯化的水解产物中，可以通过LC-MS鉴定20种不同的α-酪蛋白（CN）衍生的肽。大多数肽来自β-酪蛋白的中央和C末端区域。肽长度在9（β-CN（F 59-67））和22个氨基酸（β-CN（F 117-138）之间）。

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