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首页> 外文期刊>Anticancer Research: International Journal of Cancer Research and Treatment >Papuamine Inhibits Viability of Non-small Cell Lung Cancer Cells by Inducing Mitochondrial Dysfunction
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Papuamine Inhibits Viability of Non-small Cell Lung Cancer Cells by Inducing Mitochondrial Dysfunction

机译：纸蛋白抑制非小细胞肺癌细胞的活力来诱导线粒体功能障碍

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Background/Aim: Despite the Warburg effect, mitochondria play an essential role in the survival and maintenance of cancer cells. Thus, mitochondria have been considered a target for anticancer agents. Here, we identified a mitochondria-targeting anticancer agent from natural products. Materials and Methods: Morphological and functional changes in mitochondria were determined by a fluorescence-based High Content Imaging System. Using human non-small cell lung cancer (NSCLC) cell lines (H1299, H226B, and A549), cell viability and colony formation assays, cell cycle analysis, and immunoblotting were performed to determine cytotoxic and proapoptotic effects of papuamine. Results: Using a natural product chemical library, we identified papuamine as an active compound to inhibit viability and ATP production of NSCLC cells. Papuamine depleted intracellular ATP by causing mitochondrial dysfunction, as indicated by the loss of the mitochondrial membrane potential and increased mitochondrial superoxide generation. Papuamine significantly inhibited viability and colony formation of NSCLC cells by inducing apoptosis. Conclusion: Papuamine has a potential as a novel mitochondria-targeting anticancer agent.
机译：背景/目的：尽管Warburg效应，线粒体在癌细胞的存活和维持中发挥着重要作用。因此，线粒体被认为是抗癌剂的靶标。在这里，我们鉴定了来自天然产物的线粒体靶向抗癌剂。材料和方法：通过荧光的高含量成像系统确定线粒体的形态学和功能变化。使用人的非小细胞肺癌（NSCLC）细胞系（H1299，H226B和A549），进行细胞活力和菌落形成测定，进行细胞循环分析和免疫印迹，以确定蛋白石的细胞毒性和凋亡效应。结果：采用天然产物化学文库，我们将纸黄瓜作为活性化合物鉴定，以抑制NSClC细胞的活力和ATP产生。通过引起线粒体功能障碍引起细胞内功能障碍，如线粒体膜电位的损失和增加的线粒体超氧化物产生所示，蛋白石耗尽细胞内ATP。蛋白石通过诱导细胞凋亡显着抑制NSCLC细胞的活力和菌落形成。结论：纸巾具有作为一种新型线粒体靶向抗癌剂的潜力。

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