...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Anticancer Research: International Journal of Cancer Research and Treatment >Chronic Sulforaphane Application Does Not Induce Resistance in Renal Cell Carcinoma Cells
【24h】

Chronic Sulforaphane Application Does Not Induce Resistance in Renal Cell Carcinoma Cells

机译：慢性嗜睡应用不会诱导肾细胞癌细胞的抗性

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Background/Aim: Since the natural compound sulforaphane (SFN) has been shown to stop tumor growth, renal cell carcinoma (RCC) patients often use this drug in addition to their prescribed oncotherapy. The aim of this study was to examine whether resistance to SFN may develop after long-term application. Materials and Methods: Several RCC cell lines were incubated with SFN for short periods of time (24-72 h) or long periods of time (8 weeks) and cell growth, proliferation, and cell-cycle proteins were analyzed. Results: Both short- and long-term application of SFN distinctly reduced RCC cell growth and proliferation. However, differences in the distribution of cells in each phase of the cell cycle and in the expression of cell-cycle proteins were apparent. Short-term treatment induced S-phase arrest, whereas long-term treatment induced G(0)/G(1)-phase arrest. Expression of Cdk1 and Cdk2 increased over short-term incubation, but decreased long-term. Expression of pCdk2, Akt, and Raptor were reduced following long-term SFN-exposure, but remained unchanged when SFN was applied for short periods of time. Conclusion: Chronic use of SFN did not evoke resistance, but differentially altered signaling pathways, compared to short-term use.
机译：背景/目的：由于已显示天然化合物嗜睡（SFN）以阻止肿瘤生长，肾细胞癌（RCC）患者通常除了规定的疗法外，常常使用该药物。本研究的目的是检查在长期应用后是否可能发生对SFN的抵抗力。材料和方法：分析了几个rcc细胞系与SFN孵育短时间（24-72小时）或长时间（8周）和细胞生长，增殖和细胞周期蛋白。结果：SFN的短期和长期施用明显降低了RCC细胞生长和增殖。然而，在细胞周期的每相和表达细胞周期蛋白的表达中，细胞分布的差异是显而易见的。短期治疗诱导的S相阻滞，而长期治疗诱导G（0）/ g（1） - 异节逮捕。 CDK1和CDK2的表达在短期孵育中增加，但长期下降。在长期SFN暴露之后，PCDK2，AKT和猛杆的表达减少，但是当SFN施加短时间时保持不变。结论：与短期使用相比，SFN的慢性用途没有唤起抗性，但差异改变的信号通路。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号