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首页> 外文期刊>Anticancer Research: International Journal of Cancer Research and Treatment >The Antitumor Effects of Metformin on Gastric Cancer In Vitro and on Peritoneal Metastasis
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The Antitumor Effects of Metformin on Gastric Cancer In Vitro and on Peritoneal Metastasis

机译：二甲双胍对体外胃癌的抗肿瘤作用及腹膜转移

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Background/Aim: Gastric cancer (GC) with peritoneal metastasis remains difficult to treat. The antidiabetic drug metformin exerts various antitumor effects via the 5'-adenosine monophosphate-activated protein kinase (AMPK) pathway and nuclear factor-kappa B (NF-kappa B). Therefore, we evaluated the antitumor effects of metformin for GC in vitro and on peritoneal metastasis. Materials and Methods: The human GC cell lines MKN1, MKN45, KATO-III and SNU-1 were used. The antiproliferative effect was evaluated in vitro with 0.5 mM or 25 mM glucose and in vivo using tumor xenograft peritoneal models of metastasis. The protein expression of AMPK, liver kinase B1 (LKB1) and NF-kappa B in tumors was examined by western blotting. Results: Metformin inhibited cell proliferation in all GC lines and sensitivity was increased under low-glucose conditions in vitro. Metformin also suppressed peritoneal metastasis. In tumors, metformin reduced the numbers of proliferating cells and NF-kappa B expression, but a similar trend was not noted for AMPK. Conclusion: Metformin may be a useful drug for the treatment of GC with peritoneal metastasis.
机译：背景/目的：具有腹膜转移的胃癌（GC）仍然难以治疗。抗糖尿病药物二甲双胍通过5'-腺苷的单磷酸活性蛋白激酶（AMPK）途径和核因子-Kappa B（NF-Kappa B）施加各种抗肿瘤作用。因此，我们在体外和腹膜转移中评估了Metformin对GC的抗肿瘤作用。材料和方法：使用人GC细胞系MKN1，MKN45，KATO-III和SNU-1。使用0.5mm或25mm的葡萄糖和体内使用肿瘤异种移植腹膜转移进行抗增殖效应。通过蛋白质印迹检查肿瘤中AMPK，肝激酶B1（LKB1）和NF-κB的蛋白质表达。结果：二甲双胍在体外低葡萄糖条件下抑制了所有GC线和敏感性的细胞增殖。二甲双胍也抑制了腹膜转移。在肿瘤中，二甲双胍减少了增殖细胞和NF-κB表达的数量，但AMPK未注意到类似的趋势。结论：二甲双胍可以是治疗GC的有用药物，腹膜转移。

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