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首页> 外文期刊>Anticancer Research: International Journal of Cancer Research and Treatment >Bidirectional Regulation of COX-2 Expression Between Cancer Cells and Macrophages
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Bidirectional Regulation of COX-2 Expression Between Cancer Cells and Macrophages

机译：双向调节癌细胞和巨噬细胞之间的COX-2表达

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Background/Aim: Our aim was to investigate the crosstalk between tumor and immune cells (M2 macrophages) and its effects on cyclo-oxygenase-2 (COX2) regulation in canine mammary tumors (CMT). Materials and Methods: Shlb CMT cells and human BT474 mammary or HT29 colon cancer cells were co-cultured with canine peripheral blood mononuclear cells (PBMCs) or with macrophage-like differentiated THP1 monocytes (dTHP1). Intracellular COX2 expression by PBMCs, dTHP1 and cancer cells was evaluated by flow cytometry. Results: Co-culturing of Shlb and canine PBMCs induced COX2 overexpression in CMT cells. In turn, COX2 expression by PBMCs, mostly CD68 + macrophages, was attenuated by coculture with Shlb (p=0.0001). In accordance, co-culture with dTHP1 prompted intracellular production of COX2 in both Shlb CMT cells and HT29 human colon cancer cells and reduced production of COX2 in BT474 human mammary cancer cells. The intracellular COX2 expression from dTHP1 decreased when treated with conditioned medium from cultured Shlb and HT29 cancer cells. Conclusion: Bidirectional COX2 regulation between cancer and monocytes/macrophages might shape a tolerogenic tumor microenvironment in CMT.
机译：背景/目的：我们的宗旨是探讨肿瘤和免疫细胞（M2巨噬细胞）之间的串扰及其对犬乳腺肿瘤的环氧氧酶-2（COX2）调节的影响（CMT）。材料和方法：SHLB CMT细胞和人BT474乳腺或HT29结肠癌细胞与犬外周血单核细胞（PBMC）或巨噬细胞状分化的THP1单核细胞（DTHP1）共培养。通过流式细胞术评价PBMC，DTHP1和癌细胞的细胞内COX2表达。结果：SHLB和甘氨酸PBMCS在CMT细胞中诱导COX2过表达的共培养。反过来，PBMC的COX2表达，主要是CD68 +巨噬细胞，通过与SHLB的共培育（P = 0.0001）衰减。根据，具有DTHP1的共同培养促进SHLB CMT细胞和HT29人结肠癌细胞中的COX2的细胞内产生，并降低了BT474人乳腺癌细胞中的COX2的产生。当用来自培养的SHLB和HT29癌细胞的条件培养基处理时，来自DTHP1的细胞内COX2表达降低。结论：癌症和单核细胞/巨噬细胞之间的双向COX2调节可能在CMT中塑造耐受性肿瘤微环境。

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