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首页> 外文期刊>Anticancer Research: International Journal of Cancer Research and Treatment >Usefulness of Flow Cytometry in Diagnosis of IgG4-Related Ophthalmic Disease and Extranodal Marginal Zone B-Cell Lymphoma of the Ocular Adnexa
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Usefulness of Flow Cytometry in Diagnosis of IgG4-Related Ophthalmic Disease and Extranodal Marginal Zone B-Cell Lymphoma of the Ocular Adnexa

机译：流式细胞术在IgG4相关眼科病的诊断中的有用性和眼Adnexa的胚胎边缘区B细胞淋巴瘤

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Background/Aim: Although flow cytometry (FCM) is used to evaluate cell surface markers of various leucocyte populations quantitatively, little is known about the usefulness of FCM in lymphoproliferative disorders of the ocular adnexa. The aim of this study was to disclose results of FCM, which were compared among IgG4-related ophthalmic disease (IgG4-ROD), idiopathic orbital inflammation (IOI), and extranodal marginal zone B-cell lymphoma (EMZL). Materials and Methods: This is a retrospective observational study. Sixty-nine tumors comprising of 16 IgG4-ROD, 24 IOI, and 29 EMZL were enrolled in the study. All tumors, surgically excised, were diagnosed based on histopathology, immunoglobulin (Ig) heavy chain gene rearrangement, and FCM. In FCM, the percentage of T-cell markers (CD2, CD3, CD4, CD5, CD7, CD8), B-cell markers (CD10, CD19, CD20, CD23), NK cell marker (CD56) and cell surface kappa/lambda was searched based on medical records. Ig light chain restriction was evaluated from results in kappa/lambda deviation by FCM. Results: The percentage of CD2, CD3, CD4, CD7, and CD10 was significantly higher in IgG4-ROD/IOI than EMZL (p<0.05 in every factor). In contrast, CD19 and CD20 percentages were significantly greater in EMZL than IgG4-ROD/IOI (p<0.01). There was no significant difference in any marker between IgG4-ROD and IOI. Kappa-positive cells were significantly greater in EMZL than IgG4-ROD/IOI (p<0.05). In kappa/lambda deviation, false-positive was noted in 3 (7.5%) benign IgG4-ROD/IOI and false-negative was observed in 10 (34.5%) EMZL cases. Sensitivity and specificity of Ig light chain restriction were 65.5 and 92.5%, respectively. Conclusion: Analyses of cell surface markers using FCM were useful in differentiating EMZL from IgG4-ROD/IOI. Sensitivity of Ig light chain restriction was relatively low in diagnosis of EMZL using FCM.
机译：背景/目的：虽然流式细胞术（FCM）用于定量评估各种白细胞群的细胞表面标志物，但关于在眼腹腺癌淋巴抑制性疾病中FCM的有用性毫无吻。本研究的目的是公开FCM的结果，其在IgG4相关眼科疾病（IgG4-棒），特发性眶炎（IOI）和外胚间区域B细胞淋巴瘤（EMZL）中进行了比较。材料和方法：这是一个回顾性观察研究。六十九个肿瘤包含16个IgG4-棒，24只IOI和29个EMZL。手术切除的所有肿瘤均基于组织病理学，免疫球蛋白（IG）重链基因重排和FCM诊断。在FCM中，T细胞标记物（CD2，CD3，CD4，CD5，CD7，CD8），B细胞标记物（CD10，CD19，CD20，CD23），NK细胞标志物（CD56）和细胞表面κ/ Lambda的百分比根据病历搜索。从FCM的Kappa / Lambda偏差的结果评估IG轻链限制。结果：IgG4-棒/ IOI的CD2，CD3，CD4，CD7和CD10的百分比显着高于EMZL（每个因素的P <0.05）。相比之下，EMZL的CD19和CD20百分比比IgG4-棒/ IOI显着更大（P <0.01）。 IgG4-棒与IOI之间的任何标记没有显着差异。在EMZL的kappa阳性细胞比IgG4-棒/ IOI显着更大（P <0.05）。在Kappa / Lambda偏差中，假阳性在3（7.5％）良性IgG4-棒/ IOI和假阴性在10（34.5％）EMZL病例中。 IG轻链限制的敏感性和特异性分别为65.5和92.5％。结论：使用FCM的细胞表面标志物分析可用于区分IgG4-棒/ IOI的Emz1。 IG轻链限制的敏感性在使用FCM的EMZL诊断中相对较低。

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