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首页> 外文期刊>Antimicrobial agents and chemotherapy. >Genomic and Molecular Characterization of Clinical Isolates of Enterobacteriaceae Harboring mcr-1 in Colombia, 2002 to 2016
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Genomic and Molecular Characterization of Clinical Isolates of Enterobacteriaceae Harboring mcr-1 in Colombia, 2002 to 2016

机译：肠杆菌植物植物临床分离物的基因组和分子表征MCR-1在哥伦比亚，2002至2016年

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Polymyxins are last-resort antimicrobial agents used to treat infections caused by carbapenem-resistant Enterobacteriaceae. Due to the worldwide dissemination of polymyxin resistance in animal and human isolates, we aimed to characterize polymyxin resistance associated with the presence of mcr-1 in Enterobacteriaceae and nonfermenter Gram-negative bacilli, using isolates collected retrospectively in Colombia from 2002 to 2016. A total of 5,887 Gram-negative clinical isolates were studied, and 513 were found to be resistant to the polymyxins. Susceptibility to colistin was confirmed by broth microdilution for all mcr-1-positive isolates, and these were further subjected to whole-genome sequencing (WGS). The localization of mcr-1 was confirmed by S1 pulsed-field gel electrophoresis (S1-PFGE) and CeuI-PFGE hybridization. Transferability was evaluated by mating assays. A total of 12 colistin-resistant isolates recovered after 2013 harbored mcr-1, including 8 Escherichia coli, 3 Salmonella enterica serovar Typhimurium, and 1 Klebsiella pneumoniae isolate. E. coli isolates were unrelated by PFGE and belonged to 7 different sequence types (STs) and phylogroups. S. Typhimurium and K. pneumoniae isolates belonged to ST34 and ST307, respectively. The mcr-1 gene was plasmid borne in all isolates but two E. coli isolates which harbored it on the chromosome. Conjugation of mcr-1 was successful in 8 of 10 isolates (8.2 x 10(-5) to 2.07 x 10(-1) cell per recipient). Plasmid sequences showed that the mcr-1 plasmids belonged to four different Inc groups (a new IncP-1 variant and the IncFII, IncHI1, and IncH families). Our results indicate that mcr-1 is circulating in clinical isolates of colistin-resistant Enterobacteriaceae in Colombia and is mainly harbored in transferable plasmids.
机译：多元辛是用于治疗Carbapenem抗生素痤疮造成的感染的最后一段抗菌剂。由于全球传播动物和人类分离物中的多脂素抗性，我们的目的是在2002年至2016年的哥伦比亚中回顾性收集的分离物，表征了与肠杆菌和革兰氏阴性的存在相关的多辛含量抗性相关。研究了5,887克阴性临床分离株，发现513个耐多酸辛。通过对所有MCR-1阳性分离株的肉汤微脱液确认对Colistin的易感性，并进一步进行全基因组测序（WGS）。通过S1脉冲场凝胶电泳（S1-PFGE）和Ceui-PFGE杂交来证实MCR-1的定位。通过配合测定评估可转移性。在2013年后，共回收了12个耐菌抗性分离物，其中包括8个大肠杆菌，3种大肠杆菌，3个沙门氏菌肠道毒素伤寒毒蕈，和1个Klebsiella肺炎肠道分离物。大肠杆菌分离株通过PFGE不相关，属于7种不同的序列类型（STS）和晶粒。 S. Typhimurium和K.肺炎群岛分别属于ST34和ST307。 MCR-1基因在所有分离株中载有质粒，但两种大肠杆菌分离物在染色体上覆盖。 MCR-1的缀合在10个分离物中的8个（8.2×10（-5）至2.07×10（-1）细胞中成功）。质粒序列表明，MCR-1质粒属于四种不同的Inc基团（新的INCP-1变体和INCFII，INCFII，英寸家族）。我们的结果表明，MCR-1在哥伦比亚耐药肠杆菌菌的临床分离株中循环，主要是在可转移质粒中呈现。

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