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首页> 外文期刊>Aquaculture >Molecular characterization of OSR1 in Pinctada fucata martensii and association of allelic variants with growth traits
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Molecular characterization of OSR1 in Pinctada fucata martensii and association of allelic variants with growth traits

机译:澳元欧欧欧欧欧欧欧元欧妇女核苷酸的分子表征及生长性状的等位基因变异关联

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摘要

Odd-skipped related 1 (OSR1) from Pinctada fucata martensii (PmOSR1) was cloned in this study. PmOSR1 contained an open reading frame measuring 951 bp that encoded a polypeptide of 316 residues. Domain analysis also revealed that PmOSR1 had a typical ZnF_C2H2 domain, indicating high identity with OSR1 protein sequence from Crassostrea gigas (55.25%) and Mizuhopecten yessoensis (65.48%). The expression pattern analysis of PmOSR1 revealed its wide expression in the tissues and developmental stages. PmOSR1 also presented a higher expression in the fast-growing group and hybrid families than in the slow-growing group and inbred families. The polymorphism analysis of PmOSR1 revealed 45 single nucleotide polymorphisms (SNPs) in the genomic sequence, and association analysis presented four SNPs significantly associated with growth traits (namely, shell length, width, height, and shell weight; P < 0.05). Three SNPs out of the four SNPs were shaped as a haploblock, and haplotype AGT showed significantly higher growth traits than that of haplotype AACAA. Therefore, PmOSR1 could be used as a growth candidate gene for the selective breeding of P. f. martensii.
机译:在本研究中克隆了来自Pinctada Fucata Martensii(PMOSR1)的奇数跳过相关的1(OSR1)。 PMOSR1含有测量951bp的开放阅读框,其编码了316个残基的多肽。结构域分析还显示PMOSR1具有典型的ZnF_C2H2结构域,从Crassostra Gigas(55.25%)和Mizuhopecten yessoensis(65.48%)中表明与OSR1蛋白质序列的高同型。 PMOSR1的表达模式分析揭示了组织和发育阶段的广泛表达。 PMOSR1还呈现出快速生长的组和杂交家庭的表达,而不是在生长缓慢的群体和近交家庭中。 PMOSR1的多态性分析揭示了基因组序列中的45个单核苷酸多态性(SNP),结合分析呈现出与生长性状显着相关的四种SNP(即壳长,宽度,高度和壳体重量; P <0.05)。四个SNP中的三个SNP被形状为单加块,单倍型Agt显示出比单倍型Aacaa的生长性能显着较高。因此,PMOSR1可以用作生长候选基因,用于选择性育种p. f。 Martensii。

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