Peculiarities in Activation of Hydrolytic Activity of Elongation Factors

A. Paleskava; M. Yu. Kaiumov; S. V. Kirillov; A. L. Konevega

首页> 外文期刊>Biochemistry >Peculiarities in Activation of Hydrolytic Activity of Elongation Factors

【24h】

Peculiarities in Activation of Hydrolytic Activity of Elongation Factors

机译：激活伸长因子水解活性的特性

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

Translational GTPases (trGTPases) belong to the family of G proteins and play key roles at all stages of protein biosynthesis on the ribosome. Unidirectional and cyclic functioning of G proteins is ensured by their ability to switch between the active and inactive states due to GTP hydrolysis accelerated by the auxiliary GTPase-activating proteins. Although trGTPases interact with the ribosomes in different conformational states, they bind to the same conserved region, which, unlike in classical GTPase-activating proteins, is represented by ribosomal RNA. The resulting catalytic sites have almost identical structure in all elongation factors suggesting a common mechanism of GTP hydrolysis. However, fine details of the activated state formation and significantly different rates of GTP hydrolysis indicate the existence of distinctive features upon GTP hydrolysis catalyzed by the different factors. Here, we present a contemporary view on the mechanism of GTPase activation and GTP hydrolysis by the elongation factors EF-Tu, EF-G, and SelB based on the analysis of structural, biochemical, and bioinformatics data.

机译：翻译GTP酶（TRGTP酶）属于G蛋白的家族，并在核糖体上的蛋白质生物合成的所有阶段发挥关键作用。通过通过助剂GTP酶活化蛋白加速的GTP水解，通过它们在活性和无活性状态之间切换的能力来确保G蛋白的单向和环状功能。虽然TRGPASE与不同构象状态相互作用，但它们与相同的保守区域结合，其与典型GTP酶活化蛋白不同，其与核糖体RNA表示。所得催化位点在所有伸长因子中具有几乎相同的结构，表明GTP水解的常见机制。然而，活化状态形成的细节和GTP水解的显着不同的速率表明，在由不同因素催化的GTP水解时存在独特特征。在这里，我们基于结构，生物化学和生物信息学数据的分析，延长因子EF-TU，EF-G和SELB的GTP酶活化和GTP水解机制的现代观点。

著录项

来源
《Biochemistry》 |2020年第11期|共12页
作者
A. Paleskava; M. Yu. Kaiumov; S. V. Kirillov; A. L. Konevega;
展开▼
作者单位

Petersburg Nuclear Physics Institute named by B.P. Konstantinov of NRC “Kurchatov Institute”;

Petersburg Nuclear Physics Institute named by B.P. Konstantinov of NRC “Kurchatov Institute”;

Petersburg Nuclear Physics Institute named by B.P. Konstantinov of NRC “Kurchatov Institute”;

Petersburg Nuclear Physics Institute named by B.P. Konstantinov of NRC “Kurchatov Institute”;

展开▼
收录信息
原文格式 PDF
正文语种 eng
中图分类生物化学;
关键词
elongation factor; GTP hydrolysis; EF-Tu; EF-G; SelB; translation; ribosome;

机译：伸长因子;GTP水解;EF-Tu;EF-G;SELB;翻译;核糖体;

相似文献

外文文献
中文文献
专利

1. Sustained AMP-activated protein kinase activation attenuates the activity of brain-derived neurotrophic factor/tyrosine kinase receptor B signaling in mice exposed to chronic stress [J] . Li-Tao Yi, Shuang-Shuang Wang, Jie Cheng, 中华医学杂志（英文版） . 2021,第015期
2. Human pharmacokinetics of ginkgo terpene lactones and impact of carboxylation in blood on their platelet-activating factor antagonistic activity [J] . Xin-wei Liu, Fei-fei Du, Chen-chun Zhong, 中国药理学报：英文版 . 2018,第012期
3. mTORC2 promotes type Ⅰ insulin-like growth factor receptor and insulin receptor activation through the tyrosine kinase activity of mTOR [J] . Yancun Yin, Hui Hua, Minjing Li, 细胞研究（英文版） . 2016,第001期
4. Nerve Growth Factor Prevents the Apoptosis-associated Increase in Acetylcholinesterase Activity after Hydrogen Peroxide Treatment by Activating Akt [J] . Hua JIANG, Jingya ZHANG, Hui ZHU, 生物化学与生物物理学报：英文版 . 2007,第001期
5. Relationship between elongation factor 1- and elongation factor 2-dependent guanosine triphosphatase activities of ribosomes. Inhibition of both activities by ricin [J] . A Mattioli, G Testoni, L Montanaro, The biochemical journal . 1975,第3期

机译：核糖体的延伸因子1-和延伸因子2-依赖性鸟苷三磷酸酶活性之间的关系。蓖麻毒素抑制两种活性
6. Thiostrepton inhibits stable 70S ribosome binding and ribosome-dependent GTPase activation of elongation factor G and elongation factor 4 [J] . Geoff Traeger, Justin D. Walter, Margaret Hunter, Nucleic acids research . 2012,第1期

机译：硫排蛋白抑制延伸因子G和延伸因子4的稳定的70S核糖体结合和依赖核糖体的GTPase活化
7. Thiostrepton inhibits stable 70S ribosome binding and ribosome-dependent GTPase activation of elongation factor G and elongation factor 4 [J] . Walter Justin D., Hunter Margaret, Cobb Melanie, Nucleic Acids Research . 2012,第1期

机译：硫排蛋白抑制稳定的70S核糖体结合和依赖于核糖体的GTPase激活伸长因子G和伸长因子4
8. Study on the inhibiting factors of the activated sludge activity of hexavalent chromium based on the change of dissolved oxygen [C] . Wen-Yu Zhao, Zheng Zhao, Mei-Juan Xu, Annual International Forum on Energy, Environmental and Sustainable Ecosystem Development . 2017

机译：基于溶解氧变化的六价铬活性污泥活性抑制因子研究
9. Skeletal muscle eukaryotic elongation factor 2 (eEF2) response to acute resistance exercise in young and old men and women: Relationship to muscle glycogen content and 5'-AMP-activated protein kinase (AMPK) activity. [D] . Macesich, Jennifer Lea. 2010

机译：骨骼肌真核伸长因子2（eEF2）对男女青年急性抵抗运动的反应：与肌肉糖原含量和5'-AMP激活的蛋白激酶（AMPK）活性的关系。
10. N-methyl-d-aspartate receptor activation and visual activity induce elongation factor-2 phosphorylation in amphibian tecta: A role for N-methyl-d-aspartate receptors in controlling protein synthesis [O] . A. J. Scheetz, Angus C. Nairn, Martha Constantine-Paton 1997

机译：N-甲基-d-天冬氨酸受体的激活和视觉活动诱导两栖动物的伸长因子-2磷酸化：N-甲基-d-天冬氨酸受体在控制蛋白质合成中的作用
11. Interaction of elongation factor-1 alpha and pleckstrin homology domain of phospholipase C-gamma 1 with activating its activity [O] . Chang, JS, Seok, H, Kwon, TK, 2014

机译：磷脂酶C-γ1的延伸因子1α和pleckstrin同源结构域与其活性的相互作用
12. Hypoxia Inducible Factor 1 (HIF-1) Activation in U87 Glioma Cells Involves a Decrease in Reactive Oxygen Species Production and Protein Kinase C Activity [R] . Forbes, R. A. 1998

机译：U87神经胶质瘤细胞中的缺氧诱导因子1（HIF-1）活化涉及活性氧生成和蛋白激酶C活性的降低

Peculiarities in Activation of Hydrolytic Activity of Elongation Factors

摘要

著录项

相似文献

相关主题

期刊订阅