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The shape-effect of calcium phosphate nanoparticle based films on their osteogenic properties

机译：磷酸钙纳米粒子膜对其成骨特性的形状效应

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Calcium phosphates (CaPs) in the form of hydroxyapatite (HA) have been extensively studied in the context of bone regeneration due to their chemical similarity to natural bone mineral. While HA is known to promote osteogenic differentiation, the structural properties of the ceramic have been shown to affect the extent of this effect; several studies have suggested that nanostructured HA can improve the bioactivity. However, the role shape plays in the osteogenic potential is more elusive. Here we studied the effect of HA nanoparticle shape on the ability to induce osteogenesis in human mesenchymal stromal cells (hMSCs) by developing nanoparticle films using needle-, rice- and spherical-shaped HA. We showed that the HA films made from all three shapes of nanoparticles induced increased levels of osteogenic markers (i.e. runt-related transcription factor 2 (RUNX2), bone morphogenetic protein 2 (BMP2), alkaline phosphatase (ALP), osteopontin (OPN), osteocalcin (OCN) on protein and gene level in comparison to hMSCs cultured on cover glass slides. Furthermore, their expression levels and profiles differed significantly as a function of nanoparticle shape. We also showed that nanoparticle films were more efficient in inducing osteogenic gene expression in hMSCs compared to adding nanoparticles to hMSCs in culture media. Finally, we demonstrated that hMSC morphology upon adhesion to the HA nanoparticle films is dependent on nanoparticle shape, with hMSCs exhibiting a more spread morphology on needle-shaped nanoparticle films compared to hMSCs seeded on rice- and spherical-shaped nanoparticle films. Our data suggests that HA nanoparticle films are efficient in inducing hMSC osteogenesis in basic cell culture conditions and that nanoparticle shape plays a vital role in cell adhesion and morphology and extent of induction of osteogenic differentiation.
机译：由于它们与天然骨矿物的化学相似性，在骨再生的背景下已经广泛研究了羟基磷灰石（HA）形式的磷酸钙（帽）。众所周知，虽然HA促进了骨质发生分化，但已显示陶瓷的结构性能影响这种效果的程度;几项研究表明纳米结构HA可以改善生物活性。然而，在成骨潜力中发挥作用形状更难以捉摸。在这里，我们研究了HA纳米颗粒形状对使用针头，水稻和球形HA产生纳米颗粒膜的人间充质基质细胞（HMSCs）诱导骨质发生的能力。我们表明，由所有三种纳米颗粒制成的HA薄膜诱导骨质发生标记的增加（即Runt相关转录因子2（Runx2），骨形态发生蛋白2（BMP2），碱性磷酸酶（ALP），骨膨胀蛋白（OPN），与覆盖玻璃载玻片上培养的HMSCs相比蛋白质和基因水平的骨钙蛋白（OCN）。此外，它们的表达水平和曲线随纳米颗粒形状的函数显着不同。我们还表明纳米粒子薄膜在诱导骨质发生基因表达方面更有效HMSCs与将纳米颗粒添加到培养基中的HMSC。最后，我们证明了与HA纳米粒子膜的粘附后的HMSC形态取决于纳米颗粒形状，与在水稻上播种的HMSCS相比，HMSCs在针状纳米粒子膜上表现出更散发的形态 - 和球形纳米粒子膜。我们的数据表明，HA纳米粒子薄膜在诱导HMSC骨质原粒方面是有效的S在基本细胞培养条件下，纳米粒子形状在细胞粘附和形态和诱导方框分化的形态和程度上起着至关重要的作用。

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  • 来源
    《Biomaterials Science》 |2021年第5期|共13页
  • 作者

    Pichaporn Sutthavas; Pamela Habibovic; Sabine H. van Rijt;

  • 作者单位

    Department of Instructive Biomaterials Engineering MERLN Institute for Technology-Inspired Regenerative Medicine Maastricht University P.O. Box 616 6200 MD Maastricht The Netherlands;

    Department of Instructive Biomaterials Engineering MERLN Institute for Technology-Inspired Regenerative Medicine Maastricht University P.O. Box 616 6200 MD Maastricht The Netherlands;

    Department of Instructive Biomaterials Engineering MERLN Institute for Technology-Inspired Regenerative Medicine Maastricht University P.O. Box 616 6200 MD Maastricht The Netherlands;

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  • 中图分类 计量学;
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