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Production of free fatty acids from switchgrass using recombinant Escherichia coli

机译：使用重组大肠杆菌的SwitchGrass生产自由脂肪酸

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Switchgrass is a promising feedstock to generate fermentable sugars required for the sustainable operation of biorefineries because of their abundant availability, easy cropping system, and high cellulosic content. The objective of this study was to investigate the potentiality of switchgrass as an alternative sugar supplier for free fatty acid (FFA) production using engineered Escherichia coli strains. Recombinant E. coli strains successfully produced FFAs using switchgrass hydrolysates. A total of about 3 g/L FFAs were attained from switchgrass hydrolysates by engineered E. coli strains. Furthermore, overall yield assessments of our bioconversion process showed that 88 and 46% of the theoretical maximal yields of glucose and xylose were attained from raw switchgrass during sugar generation. Additionally, 72% of the theoretical maximum yield of FFAs were achieved from switchgrass hydrolysates by recombinant E. coli during fermentation. These shake-flask results were successfully scaled up to a laboratory scale bioreactor with a 4 L working volume. This study demonstrated an efficient bioconversion process of switchgrass-based FFAs using an engineered microbial system for targeting fatty acid production that are secreted into the fermentation broth with associated lower downstream processing costs, which is pertinent to develop an integrated bioconversion process using lignocellulosic biomass. (c) 2017 American Institute of Chemical Engineers Biotechnol. Prog., 34:91-98, 2018
机译：SwitchGrass是一种有希望的原料，因为它们丰富的可用性，容易的种植系统和高纤维素含量，产生生物归类的可持续运行所需的可发酵糖。本研究的目的是研究使用工程化大肠杆菌菌株的游离脂肪酸（FFA）生产的替代糖供应商的潜力。重组大肠杆菌菌株成功地使用Switchgrass水解产物制备了FFA。通过工程化大肠杆菌菌株从SwitchGrass水解产物获得了总共约3g / l FFA。此外，我们的生物转化过程的总产量评估表明，在糖生成期间，从原始交叉草中获得了88和46％的理论最大产量和木糖。另外，在发酵过程中，通过重组大肠杆菌从Recomombinant大肠杆菌获得FFA的理论最大收率的72％。这些摇瓶结果成功扩大到实验室等级的生物反应器，具有4升工作体积。该研究证明了使用用于靶向脂肪酸产生的脂肪酸产生的基于切换紫杉的FFA的高效生物转化过程，所述脂肪酸产生与相关的下游加工成本相关的发酵液分泌，这与使用木质纤维素生物质进行综合生物转化过程。 （c）2017美国化学工程师生物科技学院。 Prog。，34：91-98,2018

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