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首页> 外文期刊>Biomedical Chromatography: An International Journal Devoted to Research in Chromatographic Methodologies and Their Applications in the Biosciences >Simple and sensitive HPLC method for determination of amrubicin and amrubicinol in human plasma: application to a clinical pharmacokinetic study.
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Simple and sensitive HPLC method for determination of amrubicin and amrubicinol in human plasma: application to a clinical pharmacokinetic study.

机译：用于测定人血浆中氨柔比星和氨柔比星醇的简单而灵敏的HPLC方法：在临床药代动力学研究中的应用。

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摘要

A simple and sensitive high-performance liquid chromatographic (HPLC) method was developed for determination of amrubicin and its metabolite amrubicinol in human plasma. After protein precipitation with methanol without evaporation procedure, large volume samples were injected and separated by two monolithic columns with a guard column. The mobile phase consisted of tetrahydrofuran-dioxane-water (containing 2.3 mM acetic acid and 4 mM sodium 1-octanesulfonate; 2:6:15, v/v/v). Wavelengths of fluorescence detection were set at 480 nm for excitation and 550 nm for detection. Under these conditions, linearity was confirmed in the 2.5-5000 ng/mL concentration range of both compounds. The intra- and inter-day precision and intra- and inter-day accuracy for both compounds were less than 10%. The method was successfully applied to a clinical pharmacokinetic study of amrubicin and amrubicinol in cancer patients.
机译：建立了一种简单灵敏的高效液相色谱法（HPLC），用于测定人血浆中的氨柔比星及其代谢物氨柔比星醇。在没有蒸发步骤的情况下用甲醇沉淀蛋白质后，将大体积样品注入并通过两个带有保护柱的整体柱进行分离。流动相由四氢呋喃-二恶烷-水（含2.3 mM乙酸和4 mM 1-辛烷磺酸钠; 2：6：15，v / v / v）组成。荧光检测的波长设置为激发波长为480 nm，检测波长为550 nm。在这些条件下，两种化合物的浓度在2.5-5000 ng / mL范围内均确认为线性。两种化合物的日间和日间精度以及日间和日间精度均小于10％。该方法已成功地应用于氨柔比星和氨柔比星在癌症患者中的临床药代动力学研究。

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