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Expansion of retinal stem cells and their progeny using cell microcarriers in a bioreactor

机译：在生物反应器中使用细胞微载体的视网膜干细胞及其后代的扩增

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Abstract > Blindness as a consequence of degenerative eye diseases (e.g., age‐related macular degeneration and retinitis pigmentosa) is a major health problem and numbers are expected to increase by up to 50% by 2020. Unfortunately, adult mouse and human retinal stem cells (RSCs), unlike fish and amphibians , are quiescent in vivo and do not regenerate following disease or injury. To replace lost cells, we used microcarriers (MCs) in a suspension stirring bioreactor to help achieve numbers suitable for differentiation and transplantation. We achieved a significant 10‐fold enrichment of RSC yield compared to conventional static culture techniques using a combination of FACTIII MCs and relative hypoxia (5%) inside the bioreactor. We found that hypoxia (5% O <sub>2</sub> ) was associated with better RSC expansion across all platforms; and this can be attributed to hypoxia‐induced increases in survival and/or symmetric division of stem cells. In the future, we will target the differentiation of RSCs and their progeny toward rod and cone photoreceptor phenotypes using FACTIII MCs inside bioreactors to expand their populations in order to produce the large numbers of cells needed for transplantation. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“Main”XML：Lang =“en”> <标题类型=“main”>抽象</ title> > 由于退行性眼部疾病（例如，年龄相关的黄斑变性和视网膜炎）的后果是一个主要的健康问题，预计到2020年的数量将增加高达50％。不幸的是，成年小鼠和人类视网膜干细胞（RSCs ），与鱼和两栖动物不同，</ b> 在体内静态，并且在疾病或伤害后不会再生。为了替换丢失的细胞，我们在悬浮液中使用微载体（MCS）搅拌生物反应器，以帮助实现适合于分化和移植的数字。与使用生物反应器内部的常规静态培养技术相比，我们达到了RSC产量的显着10倍的富集。我们发现缺氧（5％o <sub> 2 </ sub> ）与所有平台的更好的RSC扩展相关联;这可归因于干细胞的存活和/或对称分裂的缺氧诱导的增加。在未来，我们将在生物反应器内使用ImpereIIS MCS来瞄准RSC和它们的后代对杆和锥形感光表谱的差异化，以扩大其群体以产生移植所需的大量细胞。 </ p> </摘要> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-15005/'>《Biotechnology Progress》</a> <b style="margin: 0 2px;">|</b><span>2019年第3期</span><b style="margin: 0 2px;">|</b><span>共11页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Baakdhah Tahani&option=202" target="_blank" rel="nofollow">Baakdhah Tahani;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=van der Kooy Derek&option=202" target="_blank" rel="nofollow">van der Kooy Derek;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Institute of Medical ScienceUniversity of TorontoToronto Ontario Canada;</p> <p>Institute of Medical ScienceUniversity of TorontoToronto Ontario Canada;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/15.html" title="生物科学">生物科学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=bioreactor&option=203" rel="nofollow">bioreactor;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=hypoxia&option=203" rel="nofollow">hypoxia;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=microcarriers&option=203" rel="nofollow">microcarriers;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=retina&option=203" rel="nofollow">retina;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=stem cells&option=203" rel="nofollow">stem cells;</a> </p> <div class="translation"> 机译：生物反应器;缺氧;微载体;视网膜;干细胞; 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Zhai Zhimin</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Sun Zimin&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,Sun Zimin</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Li Qing&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,Li Qing</a> <span> <a href="/conference-cn-26463/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 2005年华东六省一市血液病学学术会议暨浙江省血液病学学术年会 </a> <span> <span> . 2005</span> </span> </div> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/academic-degree-domestic_mphd_thesis/020315124361.html">Research of Co-cultured of Rat Myocardial Cells and Human Umbilical Cord Mesenchymal Stem Cells</a> <b>[A] </b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=冯璇&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 冯璇</a> <span> . 2016</span> </span> </div> </li> </ul> <ul style="display: none;"> 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</div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：扩增/保存未分化的造血干细胞或祖细胞的方法，制备可用于扩增/保存未分化的造血干细胞或祖细胞的条件胃细胞培养基的方法，将未分化的造血干细胞或祖细胞移植到容器中的方法，生物反应器的缓冲液和生物反应器 </span> </p> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/patent-detail/06130424192562.html">Dimensional hollow fibre bioreactor systems for the maintenance, expansion, differentiation and harvesting of human stem cells and their progeny</a> <b>[P]</b> . <span> 外国专利：  GB201019932D0 </span> <span> . 2011-01-05</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：中空纤维生物反应器系统，用于维持，扩增，分化和收获人类干细胞及其后代 </span> </p> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/patent-detail/06130424185018.html">Microcarrier for the culture if stem cells, method of culturing stem cells and method of using said microcarrier</a> <b>[P]</b> . <span> 外国专利：  GB201105503D0 </span> <span> . 2011-05-18</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：用于干细胞培养的微载体，干细胞的培养方法和使用所述微载体的方法 </span> </p> </li> </ul> </div> </div> </div> <div class="theme cardcommon" style="overflow: auto;display:none"> <h3 class="all_title" id="enpatent55">相关主题</h3> <ul id="subject"> </ul> </div> </div> </div> </div> <div class="right rightcon"> <div class="details_img cardcommon clearfix" style="margin-bottom: 10px;display:none;" > </div> </div> </div> <div id="thesis_get_original1" class="downloadBth" style="bottom: 19px;z-index: 999;" onclick="ywcd('0704028287830','4',7,2,1,'',this,24)" class="delivery" prompt="010401" title="通过人工服务将文献原文发送至邮箱" >获取原文</div> <div class="journalsub-pop-up" style="display: none"> <div class="journal-sub"> <h2>期刊订阅</h2> <img 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