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首页> 外文期刊>Biotechnology Progress >Response surface methodology optimization of polyhydroxyalkanoate production byBurkholderia cepaciaBPT1213using waste glycerol from palm oil-based biodiesel production
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Response surface methodology optimization of polyhydroxyalkanoate production byBurkholderia cepaciaBPT1213using waste glycerol from palm oil-based biodiesel production

机译：作者：张莹莹，王莹，王莹，王莹，王莹，王莹，王莹，王莹，王莹，王莹，王莹，王莹，王莹，王莹，王莹，王莹，王莹，王玮

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摘要

The feasibility of using waste glycerol from the biodiesel industry for biosynthesis of polyhydroxyalkanoate (PHA) byBurkholderia cepaciaBPT1213 was evaluated. Culture conditions were optimized by growingB. cepaciaBPT1213 in mineral salt medium supplemented with 2% waste glycerol in a 2.5 L bioreactor. Response surface methodology was used to determine the influence of aeration rate (0.6-1.8 vvm), agitation speed (100-300 rpm), and cultivation period (48-72 hr) on PHA production. The optimum conditions for the growth and PHA accumulation were 1.5 vvm, 300 rpm, and 72 hr, with predicted values of 5.08 g/L cell dry weight (CDW), 66.07% PHA content, and 3.35 g/L total PHA concentration. Using these conditions, the experimental system produced 5.63 g/L of CDW with 64.00% wt/wt PHA content, which is threefold higher PHA concentration (3.60 g/L) compared to the non-optimized conditions. The melting temperature (T-m) of purified PHA was 173.45 +/- 1.05 degrees C. In conclusion, the statistical approach was significantly increased the PHA production using waste glycerol as the sole carbon source.
机译：评估了使用来自生物柴油业生物合成的生物柴油行业的废甘油（PHA）Byburkholderia Cepaciabpt1213的可行性。通过种植方式优化培养条件。 Cepaciabpt1213在矿物盐培养基中补充有2％废甘油的2.5升生物反应器。响应面方法用于确定通气率（0.6-1.8 VVM），搅拌速度（100-300rpm）和培养期（48-72小时）对PHA生产的影响。生长和PHA积累的最佳条件为1.5 VVM，300rpm和72小时，预测值为5.08g / l细胞干重（CdW），66.07％pHa含量和3.35g / l总PHA浓度。使用这些条件，实验系统产生5.63g / L的CDW，64.00％wt / wt pHa含量，与非优化条件相比，这是三倍的PHA浓度（3.60g / L）。纯化的PHA的熔化温度（T-M）为173.45 +/- 1.05℃。总之，使用废甘油作为唯一碳源显着增加了PHA生产的PHA生产。

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