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Scaffold-free biofabrication of adipocyte structures with magnetic levitation

机译：无支架的生物结缔组与磁悬浮的脂肪细胞结构

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摘要

Tissue engineering research aims to repair the form and/or function of impaired tissues. Tissue engineering studies mostly rely on scaffold-based techniques. However, these techniques have certain challenges, such as the selection of proper scaffold material, including mechanical properties, sterilization, and fabrication processes. As an alternative, we propose a novel scaffold-free adipose tissue biofabrication technique based on magnetic levitation. In this study, a label-free magnetic levitation technique was used to form three-dimensional (3D) scaffold-free adipocyte structures with various fabrication strategies in a microcapillary-based setup. Adipogenic-differentiated 7F2 cells and growth D1 ORL UVA stem cells were used as model cells. The morphological properties of the 3D structures of single and cocultured cells were analyzed. The developed procedure leads to the formation of different patterns of single and cocultured adipocytes without a scaffold. Our results indicated that adipocytes formed loose structures while growth cells were tightly packed during 3D culture in the magnetic levitation platform. This system has potential for ex vivo modeling of adipose tissue for drug testing and transplantation applications for cell therapy in soft tissue damage. Also, it will be possible to extend this technique to other cell and tissue types.
机译：组织工程研究旨在修复损伤组织的形式和/或功能。组织工程研究大多依赖于基于脚手架的技术。然而，这些技术具有某些挑战，例如选择适当的支架材料，包括机械性能，灭菌和制造过程。作为替代方案，我们提出了一种基于磁悬浮的新型支架无脂肪组织生物破旧技术。在该研究中，使用可标记的磁悬浮技术来形成三维（3D）无支腿的无抗脂肪细胞结构，其在基于微毛细管的设置中具有各种制造策略。使用脂肪生分化的7F2细胞和生长D1 ORL UVA干细胞作为模型细胞。分析了单和共培养细胞3D结构的形态学性质。开发的程序导致形成不同图案的单一和可携带的脂肪细胞没有支架。我们的结果表明，脂肪细胞形成松散的结构，而在磁悬浮平台的3D培养期间生长细胞紧密包装。该系统具有对脂肪组织的前体内建模的潜力，用于药物检测和移植应用在软组织损伤中进行细胞疗法。而且，可以将该技术扩展到其他细胞和组织类型。

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