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首页> 外文期刊>Caryologia: Giornale de Citologia, Citosistematica e Citogenetica >A comparative karyo-morphometric analysis of Indian landraces of Sesamum indicum using EMA-giemsa and fluorochrome banding
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A comparative karyo-morphometric analysis of Indian landraces of Sesamum indicum using EMA-giemsa and fluorochrome banding

机译：使用EMA-GIEMSA和荧光染色条件的芝麻型帝国帝国帝国体力学分析

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摘要

Sesamum indicum commonly known as 'Sesame', 'Til' or 'Gingli' is an age-old high valued oil crop. With distinct seed and floral diversity and no detailed chromosomal analysis is available on Indian landraces of S. indicum (2n= 26). The present study demonstrates standardization of enzymatic maceration and air drying (EMA) method of chromosome preparation and comparative karyometric analysis in four Indian landraces of S indicum. All the landraces were characterized by very small chromosomes, length ranging from 1.24 +/- 0.02 to 2.87 +/- 0.09 mu m. The EMA- Giemsa based karyotype analysis revealed nine pairs of chromosomes with nearly median primary constriction, three pairs were submedian and a single satellite pair in each of the studied landrace. The CMA staining of Sesamum chromosomes revealed the presence of distinct CMA positive (CMA(+ve)) signals in all the studied landraces. The Black seeded til (BT) and White seeded til (WT) were characterized by six chromosomes with distal CMA(+ve) signal on short arm, while the Dark brown seeded til (DBT) showed ten chromosomes with distal CMA(+ve) signal on short arm. The Light brown seeded til (LBT) was characterized by eight chromosomes with distal CMA(+ve) signal on short arm. The results obtained from the scatter plot of Al versus A2 and PCA analysis provide a strong relationship with that of the fluorochrome banding analysis. The present research offers an explicit karyo-morphometric characterization of four Indian landraces of S. indicum for the first time.
机译：芝麻（Sesamum indicum）俗称芝麻（Sesame）、‘Til’或‘Gingli’，是一种古老的高价值油料作物。印度野牡丹（2n=26）具有独特的种子和花多样性，没有详细的染色体分析。本研究展示了印度四个地方品种的染色体制备和比较核型分析的酶浸渍和空气干燥（EMA）方法的标准化。所有地方品种都有非常小的染色体，长度在1.24+/-0.02到2.87+/-0.09μm之间。基于EMA-Giemsa的核型分析显示，在所研究的地方品种中，有九对染色体具有几乎正中的初级收缩，三对为亚正中染色体，一对为单卫星染色体。芝麻染色体的CMA染色显示在所有研究的地方品种中都存在明显的CMA阳性（CMA（+ve））信号。黑籽til（BT）和白籽til（WT）的特征是短臂上有6条染色体具有远端CMA（+ve）信号，而深棕色籽til（DBT）的特征是短臂上有10条染色体具有远端CMA（+ve）信号。浅棕色种子til（LBT）由八条染色体组成，短臂上有远端CMA（+ve）信号。从Al与A2的散点图和PCA分析中获得的结果与荧光显带分析的结果有很强的相关性。本研究首次对印度四个地方品种进行了明确的核型形态特征描述。

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