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首页> 外文期刊>Cell metabolism >Mitochondria Bound to Lipid Droplets Have Unique Bioenergetics, Composition, and Dynamics that Support Lipid Droplet Expansion
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Mitochondria Bound to Lipid Droplets Have Unique Bioenergetics, Composition, and Dynamics that Support Lipid Droplet Expansion

机译:线粒体与脂液滴相结合,具有支持脂质液滴膨胀的独特生物植物,组成和动力学

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摘要

Mitochondria associate with lipid droplets (LDs) in fat-oxidizing tissues, but the functional role of these peridroplet mitochondria (PDM) is unknown. Microscopic observation of interscapular brown adipose tissue reveals that PDM have unique protein composition and cristae structure and remain adherent to the LD in the tissue homogenate. We developed an approach to isolate PDM based on their adherence to LDs. Comparison of purified PDM to cytoplasmic mitochondria reveals that (1) PDM have increased pyruvate oxidation, electron transport, and ATP synthesis capacities; (2) PDM have reduced beta-oxidation capacity and depart from LDs upon activation of brown adipose tissue thermogenesis and beta-oxidation; (3) PDM support LD expansion as Perilipin5-induced recruitment of mitochondria to LDs increases ATP synthase-dependent triacylglyceride synthesis; and (4) PDM maintain a distinct protein composition due to uniquely low fusion-fission dynamics. We conclude that PDM represent a segregated mitochondrial population with unique structure and function that supports triacylglyceride synthesis.
机译:线粒体与脂肪氧化组织中的脂滴(LDs)有关,但这些脂滴周围线粒体(PDM)的功能作用尚不清楚。对肩胛间棕色脂肪组织的显微镜观察表明,PDM具有独特的蛋白质组成和嵴结构,并在组织匀浆中保持与LD的粘附。我们开发了一种基于PDM对LDs的依从性来隔离PDM的方法。将纯化的PDM与细胞质线粒体进行比较发现:(1)PDM增加了丙酮酸氧化、电子传递和ATP合成能力;(2) PDM降低了β-氧化能力,并在棕色脂肪组织产热和β-氧化激活后脱离LDs;(3) PDM支持LD扩张,因为外脂蛋白5诱导线粒体募集到LDs,增加ATP合酶依赖的三酰甘油酯合成;(4)由于独特的低融合裂变动力学,PDM保持了独特的蛋白质组成。我们得出结论,PDM代表一个分离的线粒体群体,具有支持三酰甘油酯合成的独特结构和功能。

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  • 来源
    《Cell metabolism》 |2018年第4期|共15页
  • 作者单位

    Univ Calif Los Angeles David Geffen Sch Med Dept Med Div Endocrinol Los Angeles CA 90095 USA;

    Univ Calif Los Angeles David Geffen Sch Med Dept Med Div Endocrinol Los Angeles CA 90095 USA;

    Boston Univ Sch Med Dept Med Obes Res Ctr Boston MA 02118 USA;

    Univ Calif Los Angeles David Geffen Sch Med Dept Med Div Endocrinol Los Angeles CA 90095 USA;

    Karolinska Inst Dept Med Dermatol &

    Venereol Unit Stockholm Sweden;

    Univ Calif Los Angeles David Geffen Sch Med Dept Med Div Endocrinol Los Angeles CA 90095 USA;

    Univ Calif Los Angeles David Geffen Sch Med Dept Med Div Endocrinol Los Angeles CA 90095 USA;

    Univ Calif Los Angeles David Geffen Sch Med Dept Med Div Endocrinol Los Angeles CA 90095 USA;

    Univ Fed Rio de Janeiro Inst Med Biochem Leopoldo de Meis Rio De Janeiro Brazil;

    Univ Ancona Dept Expt &

    Clin Med Ancona Italy;

    Univ Maryland Sch Med Dept Med Baltimore MD 21201 USA;

    Univ Calif Los Angeles David Geffen Sch Med Dept Biol Chem Los Angeles CA 90095 USA;

    Univ Calif Los Angeles David Geffen Sch Med Dept Biol Chem Los Angeles CA 90095 USA;

    Boston Univ Sch Med Dept Med Obes Res Ctr Boston MA 02118 USA;

    Univ Calif Los Angeles David Geffen Sch Med Dept Med Div Endocrinol Los Angeles CA 90095 USA;

    Univ Calif Los Angeles David Geffen Sch Med Dept Med Div Endocrinol Los Angeles CA 90095 USA;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 内分泌腺疾病及代谢病;
  • 关键词

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