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首页> 外文期刊>Cell biology international. >Ectophosphatase activity in the triple-negative breast cancer cell line MDA-MB-231
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Ectophosphatase activity in the triple-negative breast cancer cell line MDA-MB-231

机译：三阴性乳腺癌细胞系MDA-MB-231中的异位磷酶活性

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Breast cancer is one of the most common cancers in the female population worldwide, and its development is thought to be associated with genetic mutations that lead to uncontrolled and accelerated growth of breast cells. This abnormal behavior requires extra energy, and indeed, tumor cells display a rewired energy metabolism compared to normal breast cells. Inorganic phosphate (Pi) is a glycolytic substrate of glyceraldehyde-3-phosphate dehydrogenase and has an important role in cancer cell proliferation. For cells to obtain Pi, ectoenzymes in the plasma membrane with their catalytic site facing the extracellular environment can hydrolyze phosphorylated molecules, and this is an initial and possibly limiting step for the uptake of Pi by carriers that behave as adjuvants in the process of energy harvesting and thus partially contributes to tumor energy requirements. In this study, the activity of an ectophosphatase in MDA-MB-231 cells was biochemically characterized, and the results showed that the activity of this enzyme was higher in the acidic pH range and that the enzyme had a K-m = 4.5 +/- 0.5 mM para-nitrophenylphosphate and a V-max = 2280 +/- 158 nM x h(-1) x mg protein(-1). In addition, classical acid phosphatase inhibitors, including sodium orthovanadate, decreased enzymatic activity. Sodium orthovanadate was able to inhibit ectophosphatase activity while also inhibiting cell proliferation, adhesion, and migration, which are important processes in tumor progression, especially in metastatic breast cancer MDA-MB-231 cells that have higher ectophosphatase activity than MCF-7 and MCF-10 breast cells.
机译：乳腺癌是全世界女性人群中最常见的癌症之一，其发展被认为与导致乳腺细胞不受控制和加速生长的基因突变有关。这种异常的行为需要额外的能量，事实上，与正常的乳腺细胞相比，肿瘤细胞显示出重新连接的能量代谢。无机磷酸盐（Pi）是甘油醛-3-磷酸脱氢酶的糖酵解底物，在癌细胞增殖中起重要作用。为了使细胞获得Pi，质膜中的胞外酶（其催化位点面向细胞外环境）可以水解磷酸化分子，这是载体摄取Pi的初始步骤，可能是限制步骤，载体在能量收集过程中充当佐剂，从而部分促进肿瘤能量需求。在本研究中，对MDA-MB-231细胞中的一种外磷酸酯酶的活性进行了生化表征，结果表明，该酶在酸性pH范围内的活性更高，并且该酶具有K-m=4.5+/-0.5 mM对硝基苯磷酸酯和V-max=2280+/-158 nM x h（-1）x mg蛋白质（-1）。此外，包括正钒酸钠在内的经典酸性磷酸酶抑制剂会降低酶活性。正钒酸钠能够抑制胞外磷酸酶活性，同时也抑制细胞增殖、粘附和迁移，这是肿瘤进展的重要过程，尤其是转移性乳腺癌MDA-MB-231细胞，其胞外磷酸酶活性高于MCF-7和MCF-10乳腺细胞。

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