• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Cell biology international. >Peroxiredoxin ofArthrospira platensisderived short molecule YT12 influences antioxidant and anticancer activity
【24h】

Peroxiredoxin ofArthrospira platensisderived short molecule YT12 influences antioxidant and anticancer activity

机译：过氧化唑胺铂铂的短分子YT12影响抗氧化剂和抗癌活性

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

This study demonstrates both the antioxidant and anticancer potential of the novel short molecule YT12 derived from peroxiredoxin (Prx) of spirulina,Arthrospira platensis(Ap). ApPrx showed significant reduction in reactive oxygen species (ROS) against hydrogen peroxide (H2O2) stress. The complementary DNA sequence ofApPrx contained 706 nucleotides and its coding region possessed 546 nucleotides between position 115 and 660. Real-time quantitative reverse transcription polymerase chain reaction analysis confirmed the messenger RNA expression ofApPrx due to H(2)O(2)exposure in spirulina cells at regular intervals, in which the highest expression was noticed on Day 20. Cytotoxicity assay was performed using human peripheral blood mononuclear cells, and revealed that at 10 mu M, the YT12 did not exhibit any notable toxicity. Furthermore, ROS scavenging activity of YT12 was performed using DCF-DA assay, in which YT12 scavenged a significant amount of ROS at 25 mu M in H2O2-treated blood leukocytes. The intracellular ROS in human colon adenocarcinoma cells (HT-29) was regulated by oxidative stress, where the YT12 scavenges ROS in HT-29 cells at 12.5 mu M. Findings show that YT12 peptide has anticancer activity, when treated against HT-29 cells. Through the MTT assay, YT12 showed vital cytotoxicity against HT-29 cells. These finding suggested that YT12 is a potent antioxidant molecule which defends ROS against oxidative stress and plays a role in redox balance.
机译：这项研究证明了新型短分子YT12的抗氧化和抗癌潜力，YT12来源于螺旋藻的过氧化物酶（Prx），即钝顶节螺旋体（Ap）。APPX显示活性氧（ROS）在过氧化氢（H2O2）胁迫下显著减少。PPRX的互补DNA序列包含706个核苷酸，其编码区在115位和660位之间有546个核苷酸。实时定量逆转录聚合酶链反应分析证实，螺旋藻细胞定期暴露H（2）O（2）导致的APPRX信使RNA表达，其中在第20天发现最高表达。使用人类外周血单个核细胞进行细胞毒性试验，结果表明，在10μM浓度下，YT12没有表现出任何明显的毒性。此外，YT12的活性氧清除活性通过DCF-DA分析进行，其中YT12在25μM H2O2处理的血液白细胞中清除大量活性氧。人结肠腺癌细胞（HT-29）中的细胞内ROS受氧化应激的调节，其中YT12在12.5μM时清除HT-29细胞中的ROS。研究结果表明，YT12肽对HT-29细胞具有抗癌活性。通过MTT试验，YT12对HT-29细胞显示出重要的细胞毒性。这些发现表明YT12是一种有效的抗氧化分子，可以保护活性氧抵抗氧化应激，并在氧化还原平衡中发挥作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号