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首页> 外文会议>International Conference on Nanotechnologies and Biomedical Engineering >Testing Green Silver Nanoparticles for Genotoxicity, Antioxidant and Anticancer Activity
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Testing Green Silver Nanoparticles for Genotoxicity, Antioxidant and Anticancer Activity

机译：测试绿色银纳米粒子以进行遗传毒性，抗氧化和抗癌活性

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The toxicity of the synthesized nanoparticles (NPs) by various physicochemical methods is one of the main problems for their application. NPs synthesized using plant extracts are less toxic than other methods of their congregation, so, in this regard, the synthesis of this so-called "Green NPs" is very important. Direct interaction of the AgNPs obtained from the 50% ethanol extract of Ocimum araratum with the genomic DNA of sarcoma S-180 cells by the method of retardation has been investigated. No genotoxicity of the stabilized green AgNPs has been detected, which extends its use in vivo. Testing of green NPs was carried out on outbred mice with S-180 induced sarcoma. The changes in the intensity of spontaneous chemiluminescence (SChL) of the homo-genates of the tumor tissue of the S-180 sarcoma of all groups of mice were investigated. For evaluation of lipid peroxidation (POL), the formation of malonic dialdehyde (MDA) was determined using thiobarbituric acid (TBA-test), and the activity of superoxide dismutase (SOD) was measured for the activity of the endogenous antioxidant enzyme system. The highest values of SChL and MDA were observed in the experimental group, which was administered with a stabilized extract of AgNPs, compared with control animals. SOD activity increases both in the positive control group and in the experimental group. Since the formation of MDA is characteristic of lipid peroxidation reactions and the data obtained correlate with the results of SChL and SOD, it can be argued that AgNPs increase the level of ROS and cause damage of cellular structures and biopolymers, thus causing degradation of tumor tissue.
机译：通过各种物理化学方法的合成纳米颗粒（NPS）的毒性是其应用的主要问题之一。使用植物提取物合成的NPS比其会众的其他方法毒性较小，因此，在这方面，这种所谓的“绿色NPS”的合成非常重要。研究了通过通过延迟的方法，研究了从50％乙醇提取物与Sarcoma S-180细胞的基因组DNA获得的AgNP的直接相互作用。未检测到稳定的绿色AgNP的基因毒性，其在体内使用它。在具有S-180诱导的肉瘤的异常小鼠上进行了绿NPS的测试。在小鼠中的所有组的S-180肉瘤肿瘤组织的均聚genates的自发化学发光（SCHL）的强度的变化进行了研究。对于脂质过氧化（POL）的评价，使用硫酸脲酸（TBA-TEST）测定丙二醛二醛（MDA）的形成，并测量超氧化物歧化酶（SOD）的活性用于内源性抗氧化酶系统的活性。在实验组中观察到SCH1和MDA的最高值，与对照动物相比，在实验组中观察到稳定的AgNP提取物。 SOD活性在阳性对照组和实验组中增加。由于MDA的形成是脂质过氧化反应的特征，并且获得的数据与SCHL和SOD的结果相关，因此可以认为AGNP增加ROS的水平并导致细胞结构和生物聚合物的损伤，从而导致肿瘤组织的降解。

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