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首页> 外文期刊>Cell biology international. >Interleukin-38 inhibits adipogenesis and inflammatory cytokine production in 3T3-L1 preadipocytes
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Interleukin-38 inhibits adipogenesis and inflammatory cytokine production in 3T3-L1 preadipocytes

机译：白细胞介素-38抑制3T3-L1前脂肪细胞中的脂肪发生和炎症细胞因子产生

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摘要

Interleukin-38 (IL-38) is a novel member of the IL-1 cytokine family with anti-inflammatory activity. However, its effect on adipogenesis and inflammatory cytokines secretion of adipocytes in vitro has not been reported. To address whether IL-38 inhibits adipogenesis and inflammation in vitro, adipose precursor 3T3-L1 cells were cultured with or without IL-38. The morphology and size of lipid droplets in 3T3-L1 cells were measured by Oil red O staining. The mRNA expression levels of GATA-binding protein-3 (GATA-3), glucose transporter type 4 (GLUT4), peroxisome proliferator-associated receptor gamma 2, IL-1 beta, IL-6, and monocyte chemoattractant protein-1 (MCP-1) in 3T3-L1 cells were detected by real-time PCR, The contents of IL-6, IL-1 beta, and MCP-1 in 3T3-L1 cell medium supernatants were determined by enzyme-linked immunosorbent assay. IL-38 significantly decreased the number of lipid droplets in 3T3-L1 cells. IL-38 also increased GATA-3 and GLUT4 mRNA expression and inhibited IL-1 beta, IL-6, and MCP-1 secretion by 3T3-L1 cells. It is concluded that IL-38 can inhibit the differentiation of human adipocytes and inflammatory cytokine production by 3T3-L1 cells.
机译：白细胞介素-38（IL-38）是具有抗炎活性的IL-1细胞因子家族的新成员。然而，其在体外对脂肪细胞的脂肪生成和炎性细胞因子分泌的影响尚未见报道。为了研究IL-38是否在体外抑制脂肪生成和炎症，我们在有或没有IL-38的情况下培养了脂肪前体3T3-L1细胞。油红O染色法检测3T3-L1细胞内脂滴的形态和大小。实时PCR检测3T3-L1细胞中GATA结合蛋白-3（GATA-3）、葡萄糖转运蛋白4（GLUT4）、过氧化物酶体增殖物相关受体γ2、IL-1β、IL-6和单核细胞趋化蛋白-1（MCP-1）的mRNA表达水平，酶联免疫吸附测定3T3-L1细胞培养基上清液中IL-6、IL-1β和MCP-1的含量。IL-38显著减少3T3-L1细胞中的脂滴数量。IL-38还能增加GATA-3和GLUT4 mRNA的表达，并抑制3T3-L1细胞分泌IL-1β、IL-6和MCP-1。结论：IL-38可以抑制3T3-L1细胞对人脂肪细胞的分化和炎性细胞因子的产生。

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