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首页> 外文期刊>Cell and Tissue Research >Characterization and tissue distribution of neuropeptide F in the eyestalk and brain of the male giant freshwater prawn, Macrobrachium rosenbergii
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Characterization and tissue distribution of neuropeptide F in the eyestalk and brain of the male giant freshwater prawn, Macrobrachium rosenbergii

机译：雄性巨型淡水虾的眼睑术中神经肽F的特征和组织分布，大鼠罗森伯古

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摘要

We previously analyzed the central nervous system (CNS) transcriptome and found three isotypes of long neuropeptide F (MrNPF-I, -II, -III) and four isoforms of short NPF (sMrNPF) in the giant freshwater prawn, Macrobrachium rosenbergii. We now validate the complete sequences of the MrNPF-I and -II precursor proteins, which show high similarity (91-95 %) to NPFs of the penaeus shrimp (PsNPF). MrNPF-I and -II precursors share 71 % amino acid identity, whereas the mature 32-amino-acid MrNPF-I and 69-amino-acid MrNPF-II are identical, except for a 37-amino-acid insert within the middle part of the latter. Both mature MrNPFs are almost identical to PsNPF-I and -II except for four amino acids at the mid-region of the peptides. Reverse transcription plus the polymerase chain reaction revealed that transripts of MrNPF-I and -II were expressed in various parts of CNS including the eyestalk, brain and thoracic and abdominal ganglia, with the highest expression occurring in the brain and thoracic ganglia and with MrNPF-I showing five- to seven-fold higher expression than MrNPF-II. These peptides were also expressed in the midgut hindgut, and hepatopancreas, with MrNPF-I expression in the former two organs being at the same level as that in the brain and thoracic ganglia and about 4-fold higher than NPF-II. The expression of NPFs was also detected in the testes and spermatic duct but appeared much weaker in the latter. Other tissues that also expressed a considerable amount of NPF-I included the hematopoeitic tissue, heart and muscle. By immunohistochemistry, we detected MrNPFs in neurons of clusters 2, 3 and 4 and neuropils ME, MT and SG of the optic ganglia, neurons in cluster 6 and neuropils AMPN, PMPN, PT, PB and CB of the medial protocerebrum, neurons in clusters 9 and 11 and neurophils ON and OGTN of the deutocerebrum and neurons in clusters 14, 15 and 16 and neuropils TN and AnN of the tritocerebrum. Because of their high degree of conservation and strong and wide-spread expression in tissues other than CNS, we believe that, in addition to being a neuromodulator in controlling feeding, MrNPFs also play critical roles in tissue homeostasis. This should be further explored.
机译：我们之前分析了中枢神经系统（CNS）转录组，在罗氏沼虾中发现了长神经肽F的三种同型（MrNPF-I、-II、-III）和短神经肽F的四种同型（sMrNPF）。我们现在验证了MrNPF-I和-II前体蛋白的完整序列，它们与对虾（PsNPF）的NPF具有高度的相似性（91-95%）。MrNPF-I和-II前体具有71%的氨基酸同源性，而成熟的32个氨基酸MrNPF-I和69个氨基酸MrNPF-II是相同的，只是后者中间插入了37个氨基酸。两种成熟的MRNPF与PsNPF-I和-II几乎相同，只是肽的中间区域有四种氨基酸。逆转录和多聚酶链反应显示，MrNPF-I和-II的转录产物在中枢神经系统的不同部位表达，包括眼柄、大脑、胸腹神经节，最高表达出现在大脑和胸神经节，MrNPF-I的表达比MrNPF-II高5到7倍。这些肽也在中肠、后肠和肝胰腺中表达，前两个器官中的MrNPF-I表达水平与脑和胸神经节相同，约为NPF-II的4倍。NPFs在睾丸和精索管中也有表达，但在精索管中表达较弱。其他也表达大量NPF-I的组织包括血栓性组织、心脏和肌肉。通过免疫组织化学，我们检测了视神经节第2、3和4群神经元和神经膜ME、MT和SG中的MrNPFs，中脑第6群神经元和神经膜AMPN、PMPN、PT、PB和CB，第9和11群神经元，中脑第14、15和16群神经元中的神经膜ON和OGTN，以及三头脑的神经膜TN和AnN中的神经元。由于MrNPFs具有高度的保守性，在中枢神经系统以外的组织中有强而广泛的表达，我们认为，除了作为控制摄食的神经调节剂外，MrNPFs在组织内环境稳定中也起着关键作用。这一点应该进一步探讨。

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