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首页> 外文期刊>Cell and Tissue Research >miR-23a-3p regulated by LncRNA SNHG5 suppresses the chondrogenic differentiation of human adipose-derived stem cells via targeting SOX6/SOX5
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miR-23a-3p regulated by LncRNA SNHG5 suppresses the chondrogenic differentiation of human adipose-derived stem cells via targeting SOX6/SOX5

机译：由LNCRNA SNHG5调节的miR-23a-3p通过靶向SOx6 / SOx5抑制人脂肪衍生的干细胞的软骨形成分化

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摘要

Cartilage generation and degradation are controlled by miRNAs. Our previous study showed miR-23a-3p was downregulated during chondrogenic differentiation in chondrogenic human adipose-derived mesenchymal stem cells (hADSCs). In the present study, we explored the function of miR-23a-3p in chondrogenesis differentiation. The role of miR-23a-3p in chondrogenic differentiation potential of hADSCs was assessed by Alcian blue staining, quantitative real-time polymerase chain reaction (qRT-PCR), and Western blot. We show that miR-23a-3p suppressed the chondrogenic differentiation of hADSCs. LncRNA SNHG5 interacted with miR-23a-3p, and suppression or overexpression of SNHG5 correlates with inhibition and promotion of hADSC chondrogenic differentiation, respectively. We have determined that SNHG5 can sponge miR-23a-3p to regulate the expression of SOX6/SOX5, transcription factors that play essential roles in chondrocyte differentiation. Furthermore, the overexpression of SNHG5 activates the JNK/MAPK/ERK pathway. In conclusion, miR-23a-3p regulated by lncRNA SNHG5 suppresses the chondrogenic differentiation of human adipose-derived stem cells via targeting SOX6/SOX5.
机译：软骨的生成和降解由miRNA控制。我们之前的研究表明，在成软骨的人脂肪间充质干细胞（HADSC）中，miR-23a-3p在成软骨分化过程中下调。在本研究中，我们探讨了miR-23a-3p在软骨发生分化中的作用。通过Alcian蓝染色、定量实时聚合酶链反应（qRT PCR）和Western blot评估miR-23a-3p在HADSC软骨分化潜能中的作用。我们发现miR-23a-3p抑制HADSC的软骨分化。LncRNA SNHG5与miR-23a-3p相互作用，SNHG5的抑制或过度表达分别与抑制和促进hADSC软骨分化相关。我们已经确定SNHG5可以海绵状miR-23a-3p来调节SOX6/SOX5的表达，SOX6/SOX5是在软骨细胞分化中起关键作用的转录因子。此外，SNHG5的过度表达激活了JNK/MAPK/ERK通路。总之，由lncRNA SNHG5调控的miR-23a-3p通过靶向SOX6/SOX5抑制人脂肪源性干细胞的软骨分化。

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  • 来源
    《Cell and Tissue Research》 |2021年第2期|共11页
  • 作者

    Yang Zhen; Ren Zhijing; She Rongfeng; Ao Jun; Wa Qingde; Sun Zeyu; Li Bo; Tian Xiaobin;

  • 作者单位

    Guizhou Univ Med Coll Guiyang 550025 Guizhou Peoples R China;

    Guizhou Univ Med Coll Guiyang 550025 Guizhou Peoples R China;

    Guizhou Prov Peoples Hosp Dept Orthoped Guiyang 550002 Guizhou Peoples R China;

    Zunyi Med Coll Dept Orthoped Affiliated Hosp Zunyi 563000 Guizhou Peoples R China;

    Zunyi Med Coll Dept Orthoped Affiliated Hosp Zunyi 563000 Guizhou Peoples R China;

    Guizhou Prov Peoples Hosp Dept Orthoped Guiyang 550002 Guizhou Peoples R China;

    Guizhou Prov Peoples Hosp Dept Orthoped Guiyang 550002 Guizhou Peoples R China;

    Guizhou Med Univ Dept Orthoped Affiliated Hosp Guiyang 550001 Guizhou Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 细胞生物学;
  • 关键词

    miR-23a-3p; SNHG5; Chondrogenic differentiation; hADSC; SOX6/SOX5;

    机译：miR-23a-3p;snhg5;软弱化分化;hadsc;sox6 / sox5;

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