Intranasal delivery of cationic liposome-protamine complex mRNA vaccine elicits effective anti-tumor immunity

Mai Yaping; Guo Jueshuo; Zhao Yue; Ma Shijie; Hou Yanhui; Yang Jianhong

首页> 外文期刊>Cellular immunology >Intranasal delivery of cationic liposome-protamine complex mRNA vaccine elicits effective anti-tumor immunity

【24h】

Intranasal delivery of cationic liposome-protamine complex mRNA vaccine elicits effective anti-tumor immunity

机译：阳离子脂质体 - 超蛋白复合物mRNA疫苗的鼻内递送引发有效的抗肿瘤免疫力

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

Immunization with synthetic mRNA encoding tumor-associated antigens is an emerging vaccine strategy for the treatment of cancer. In order to prevent mRNA degradation, promote antigen-presenting cells antigen presentation, and induce an anti-tumor immune response, we investigated the nasal administration of mRNA vaccines with positively charged prolamine to concentrate mRNA, form a stable polycation-mRNA complex, and encapsulate the complex with DOTAP/Chol/DSPE-PEG cationic liposomes. Cationic liposome/protamine complex (LPC) showed significantly greater efficiency in uptake of vaccine particles in vitro and stronger capacities to stimulate dendritic cell maturation, which further induced a potent anti-tumor immune response. Intranasal immunization of mice with cationic LPC containing mRNA encoding cytokeratin 19 provoked a strong cellular immune response and slowed tumor growth in an aggressive Lewis lung cancer model. The results of this study provide evidence that cationic LPC can be used as a safe and effective adjuvant and this mRNA formulation provides a basis for anti-cancer vaccination of humans.

机译：用编码肿瘤相关抗原的合成mRNA进行免疫是治疗癌症的一种新兴疫苗策略。为了防止mRNA降解，促进抗原呈递细胞抗原呈递，并诱导抗肿瘤免疫反应，我们研究了鼻腔给药带有正电荷的脯氨酸的mRNA疫苗以浓缩mRNA，形成稳定的聚阳离子mRNA复合物，并用DOTAP/Chol/DSPE-PEG阳离子脂质体包封复合物。阳离子脂质体/鱼精蛋白复合物（LPC）在体外表现出更高的疫苗颗粒摄取效率和更强的刺激树突状细胞成熟的能力，从而进一步诱导有效的抗肿瘤免疫应答。在侵袭性Lewis肺癌模型中，用含有编码细胞角蛋白19的mRNA的阳离子LPC对小鼠进行鼻内免疫，可激发强烈的细胞免疫反应并减缓肿瘤生长。这项研究的结果证明阳离子LPC可以作为一种安全有效的佐剂，这种mRNA配方为人类的抗癌疫苗接种提供了基础。

著录项

来源
《Cellular immunology》 |2020年第1期|共9页
作者
Mai Yaping; Guo Jueshuo; Zhao Yue; Ma Shijie; Hou Yanhui; Yang Jianhong;
展开▼
作者单位

Ningxia Med Univ Sch Pharm Dept Pharmaceut 1160 Shengli South St Yinchuan 750004 Ningxia;

Ningxia Med Univ Sch Pharm Dept Pharmaceut 1160 Shengli South St Yinchuan 750004 Ningxia;

Ningxia Med Univ Sch Pharm Dept Pharmaceut 1160 Shengli South St Yinchuan 750004 Ningxia;

Ningxia Med Univ Sch Pharm Dept Pharmaceut 1160 Shengli South St Yinchuan 750004 Ningxia;

Ningxia Med Univ Sch Pharm Dept Pharmaceut 1160 Shengli South St Yinchuan 750004 Ningxia;

Ningxia Med Univ Sch Pharm Dept Pharmaceut 1160 Shengli South St Yinchuan 750004 Ningxia;

展开▼
收录信息
原文格式 PDF
正文语种 eng
中图分类细胞生物学;
关键词
Cancer; Immunotherapy; Intranasal; Liposome/protamine complex; mRNA Vaccine;

机译：癌症;免疫疗法;鼻内;脂质体/ protamine络合物;mRNA疫苗;

相似文献

外文文献
中文文献
专利

1. Virus-like particle vaccine by intranasal vaccination elicits protective immunity against respiratory syncytial viral infection in mice [J] . Mengying Cai, Cheng Wang, Yufeng Li, 生物化学与生物物理学报：英文版 . 2017,第001期
2. Intranasal co-delivery of IL-6 gene enhances the immunogenicity of anti-caries DNA vaccine [J] . Ling-kai SU, Fei YU, Zhao-fei LI, 中国药理学报：英文版 . 2014,第005期
3. Humoral immune response to circulating SARS-CoV-2 variants elicited by inactivated and RBD-subunit vaccines [J] . Yunlong Cao, Tianhe Xiao, Shuo Du, 细胞研究（英文版） . 2021,第007期
4. Intratumoral delivery of an HPV vaccine elicits a broad anti-tumor immune response that translates into a potent anti-tumor effect in a preclinical murine HPV model [J] . Eiichi Ishida, Jina Lee, Jean S. Campbell, Cancer immunology, immunotherapy : . 2019,第8期

机译：HPV疫苗的妥善递送引发了一种广泛的抗肿瘤免疫应答，所述抗肿瘤免疫应答在临床前小鼠HPV模型中转化为效力的抗肿瘤作用
5. Poly (I:C)-DOTAP cationic nanoliposome containing multi-epitope HER2-derived peptide promotes vaccine-elicited anti-tumor immunity in a murine model [J] . Talesh Ghazal Alipour, Ebrahimi Zahra, Badiee Ali, Immunology Letters . 2016,第Null期

机译：包含多表位HER2衍生肽的聚（I：C）-DOTAP阳离子纳米脂质体可在小鼠模型中促进疫苗诱导的抗肿瘤免疫力
6. Therapeutic ISCOMATRIX (TM) adjuvant vaccine elicits effective anti-tumor immunity in the TRAMP-C1 mouse model of prostate cancer [J] . Barr Adele M., Silva Anabel, Prato Sandro, Cancer immunology, immunotherapy : . 2020,第10期

机译：治疗性isComatrix（TM）佐剂疫苗引发了在前列腺癌的Tramp-C1小鼠模型中有效的抗肿瘤免疫
7. HISTIDYLATED NANOVECTORS FOR MRNA VACCINE FORMULATION: INDUCTION OF A STRONG ANTI-TUMOR T CELL IMMUNITY COMBINED WITH INFLAMMATORY STATE [C] . Chantal Pichon, Patrick Midoux Nanotechnology in medicine II: briding translational in vitro and in vivo interfaces . 2018

机译：MRNA疫苗制剂的组化纳米剂：诱导强抗肿瘤T细胞免疫和炎症状态
8. Analysis of the Effects of Scheduling, Dexamethasone Co-Administration, and NKG2A Signaling on the Anti-Tumor Immune Response Elicited by Radiotherapy [D] . Battaglia, Nicholas G. 2021

机译：调度，地塞米松共同给药和NKG2A信号对放射疗法引发的抗肿瘤免疫应答的影响分析
9. Intranasal Delivery of Cationic PLGA Nano/Microparticles- Loaded FMDV DNA Vaccine Encoding IL-6 Elicited Protective Immunity against FMDV Challenge [O] . Gang Wang, Li Pan, Yongguang Zhang, 2011

机译：阳离子PLGA纳米/微粒负载的FMDV DNA疫苗的鼻内递送编码IL-6的针对FMDV挑战的保护性免疫
10. Intranasal delivery of cationic PLGA nano/microparticles-loaded FMDV DNA vaccine encoding IL-6 elicited protective immunity against FMDV challenge. [O] . Gang Wang, Li Pan, Yongguang Zhang, 2011

机译：鼻内递送负载阳离子pLGa纳米/微粒的FmDV DNa疫苗编码IL-6引发针对FmDV攻击的保护性免疫。
11. Venezuelan Equine Encephalitis Replicon Immunization Overcomes Intrinsic Tolerance and Elicits Effective Anti-Tumor Immunity to the 'Self' Tumor-Associated Antigen, neu in a Rat Mammary Tumor Model [R] . Nelson, E. L. , Prieto, D. , Alexander, T. G. , 2003

机译：委内瑞拉马脑炎复制子免疫在大鼠乳腺肿瘤模型中克服内在耐受并引发对“自身”肿瘤相关抗原，neu的有效抗肿瘤免疫

Intranasal delivery of cationic liposome-protamine complex mRNA vaccine elicits effective anti-tumor immunity

摘要

著录项

相似文献

相关主题

期刊订阅