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首页> 外文期刊>Journal of Agricultural and Food Chemistry >Transcriptomic and Metabolic Analyses Provide New Insights into the Effects of Exogenous Sucrose on Monoterpene Synthesis in 'Muscat Hamburg' Grapes
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Transcriptomic and Metabolic Analyses Provide New Insights into the Effects of Exogenous Sucrose on Monoterpene Synthesis in 'Muscat Hamburg' Grapes

机译：转录组和代谢分析为外源蔗糖对“Muscat汉堡”葡萄中的外源蔗糖的影响提供了新的见解

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摘要

Monoterpenes provide strong floral and fruit aromas to grapes and wines. In the present study, the effects of exogenous sucrose on the monoterpenes metabolites were studied at the metabolomic and transcriptomic levels in "Muscat Hamburg" grape berries. 6% sucrose aqueous solution was used as treatment, applied at the berry color change stage and 8 days after the first application. Transcriptomics analysis was carried out on berries collected at three phenological stages (berries with intermediate Brix values, E-L 36; berries not quite ripe, E-L 37; and berries harvest-ripe, E-L 38). Our results showed that the sucrose application induced the accumulation of monoterpenes at harvest, especially geraniol and geranic acid. The summary of the number of differentially expressed genes between the control and treatment was 3465, 977, and 2843 at E-L 36, E-L 37, and E-L 38, respectively. Weighted gene correlation network analysis was constructed based on the RNA-seq data, and the MElightyellow module was probably correlated with monoterpene metabolism, comprising 131 unigenes. Quantitative real-time polymerase chain expression analysis of five key differentially expressed genes in terpenoid pathways validated the RNA-seq-derived expression profiles (R-2 = 0.8143). Our findings provided new insights into the regulation of monoterpene biosynthesis in grape berries under exogenous sucrose.
机译：单萜烯为葡萄和葡萄酒提供强烈的花香和果香。本研究在代谢组学和转录组学水平上研究了外源蔗糖对“汉堡麝香”葡萄浆果中单萜类代谢产物的影响。6%蔗糖水溶液用作处理，在浆果变色阶段和首次施用后8天施用。对在三个物候期采集的浆果（具有中等锤度值的浆果，E-L 36；未完全成熟的浆果，E-L 37；成熟的浆果，E-L 38）进行转录组学分析。我们的结果表明，蔗糖施用诱导了单萜类化合物在收获时的积累，尤其是香叶醇和香叶酸。对照组和治疗组在E-L36、E-L37和E-L38的差异表达基因数量分别为3465、977和2843。基于RNA-seq数据构建加权基因相关网络分析，MElightyellow模块可能与单萜代谢相关，包含131个单基因。对萜类途径中五个关键差异表达基因的定量实时聚合酶链表达分析验证了RNA-seq衍生的表达谱（R-2=0.8143）。我们的发现为外源蔗糖对葡萄果实单萜生物合成的调控提供了新的见解。

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