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首页> 外文期刊>Journal of Chemical Education >Phytochemical Analysis and Determination of the Chemical Composition of Larvicidal Extracts of Black Pepper (Piper nigrum L.) Waste: An Undergraduate Chemistry Experiment
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Phytochemical Analysis and Determination of the Chemical Composition of Larvicidal Extracts of Black Pepper (Piper nigrum L.) Waste: An Undergraduate Chemistry Experiment

机译:黑辣椒(吹笛子Nigrum L.)废物的植物化学分析与幼虫提取物的化学成分:本科化学实验

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摘要

A laboratory experiment was developed to introduce undergraduate chemistry students to the chemical characterization of agroindustrial waste extracts by gas chromatography- mass spectrometry (GC-MS). The students prepared hexane and dichloromethane extracts from black pepper (Piper nigrum L.) waste. GC-MS analysis revealed the possible presence of sesquiterpenes and piperine, compounds commonly found in P. nigrum fruits. During postlab discussion, the students were encouraged to search for information about the biological properties of the putatively identified compounds. Most students found that piperine shows larvicidal activity. Thus, an experiment was conducted by a postgraduate research group to investigate the larvicidal potential of P. nigrum waste extracts. From the results, the students concluded that the hexane extract had higher larvicidal activity than the dichloromethane extract. We also provided students with the opportunity to identify piperine using (+)-APCI FTICR MS spectra. The proposed experiment helped students to learn and practice GC-MS and stimulated them to search the scientific literature for solutions to environmental problems.
机译:开展了一项实验室实验,向化学本科生介绍用气相色谱-质谱(GC-MS)对农业工业废物提取物进行化学表征的方法。学生们从黑胡椒(Piper nigrum L.)废料中提取己烷和二氯甲烷。GC-MS分析揭示了倍半萜和胡椒碱的可能存在,这两种化合物通常存在于黑胡椒果实中。在实验室后的讨论中,学生们被鼓励搜索关于假定已识别化合物的生物学特性的信息。大多数学生发现胡椒碱具有杀幼虫活性。因此,一个研究生研究小组进行了一项实验,以调查黑曲霉废物提取物的杀幼虫潜力。根据结果,学生们得出结论,己烷提取物比二氯甲烷提取物具有更高的杀幼虫活性。我们还为学生提供了使用(+)-APCI FTICR MS光谱识别胡椒碱的机会。拟议的实验帮助学生学习和实践GC-MS,并刺激他们搜索科学文献,寻找解决环境问题的方法。

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