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首页> 外文期刊>Journal of Medicinal Chemistry >Toward Raman Subcellular Imaging of Endothelial Dysfunction
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Toward Raman Subcellular Imaging of Endothelial Dysfunction

机译：对内皮功能障碍的拉曼亚细胞成像

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摘要

Multiple diseases are at some point associated with altered endothelial function, and endothelial dysfunction (ED) contributes to their pathophysiology. Biochemical changes of the dysfunctional endothelium are linked to various cellular organelles, including the mitochondria, endoplasmic reticulum, and nucleus, so organelle-specific insight is needed for better understanding of endothelial pathobiology. Raman imaging, which combines chemical specificity with microscopic resolution, has proved to be useful in detecting biochemical changes in ED at the cellular level. However, the detection of spectroscopic markers associated with specific cell organelles, while desirable, cannot easily be achieved by Raman imaging without labeling. This critical review summarizes the current advances in Raman-based analysis of ED, with a focus on a new approach involving molecular Raman reporters that could facilitate the study of biochemical changes in cellular organelles. Finally, imaging techniques based on both conventional spontaneous Raman scattering and the emerging technique of stimulated Raman scattering are discussed.
机译：多种疾病在某种程度上与内皮功能改变有关，内皮功能障碍（ED）是其病理生理学的一部分。功能失调的内皮细胞的生化变化与各种细胞器有关，包括线粒体、内质网和细胞核，因此需要对细胞器的特异性研究来更好地理解内皮病理生物学。拉曼成像结合了化学特异性和显微分辨率，已被证明有助于在细胞水平检测ED的生化变化。然而，与特定细胞器相关的光谱标记的检测虽然是可取的，但不需要标记就很难通过拉曼成像实现。本文综述了基于拉曼光谱的ED分析的最新进展，重点介绍了一种涉及分子拉曼报告的新方法，该方法有助于研究细胞器中的生化变化。最后，讨论了基于传统自发拉曼散射和新兴的受激拉曼散射技术的成像技术。

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